Optimization of fermentation parameters for high-activity inulinase production and purification from by Plackett-Burman and Box-Behnken.

The aim of study was to optimize fermentation parameters for inulinase production from by a statistical approach and to carry out purification of inulinase. Five isolated fungal strains were screen out inulin degradation by using Lugol's iodine solution. exhibited maximum zone of clearance around the colony and was used as an inulinase producer.

1,2-Diborolanes with strong donor substituents: Synthesis and high antimicrobial activity.

1,2-diborolanes with strong and without strong donor substituents have been described, and are also referred to as 1,2-diboracyclopentane. The 1,2-diaryl/alkyl-amino-1,2-diboracyclopentanes 2, 3, and 4 were obtained in good yield after the reaction of 1,2-dichloro-1,2-diboracyclopentane 1 with ArNHLi and MeSi-NR. The structures of these new derivatives were characterized by nuclear magnetic resonance spectroscopy.

Cloned Gene Confers Low Level Erythromycin but High Level Clindamycin Resistance in NZ131.

The most common macrolide resistance mechanisms in streptococci are the presence of methylase encoding genes and or the presence of efflux encoded by genes. In the present study we aimed to show the effects of the gene under isogenic conditions on the activities of macrolides and lincosamides in streptococci. Total DNA was extracted from C1, and the gene was amplified with or without the regulatory region using modified primer with insertion of restriction sites to clone in to pUC18.

Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247.

A total of 201 thermophilic bacteria isolated from various thermal spring, mud and soil were tested for their antibacterial activity. Among the mostly active isolates, Geobacillus toebii HBB-247 was further examined. Bacteriocin-like inhibitory substance (BLIS) produced by strain HBB-247 was found to be stable up to 60°C, sensitive to proteolytic enzymes and effective against Enterococcus faecalis, Listeria sp.

Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources.

Unlabelled: A collection of 57 enterococcal isolates from different origin (including river, treatment plant, spring and garbage water, soil, animal, and vegetables from Aydın) was screened for the production of bacteriocins. Enterococci were identified at species levels as Enterococcus faecium (34), E. hirae (6), E.