Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples.

Background: Brucellosis is a zoonotic disease caused by Brucella spp., affecting various animals and humans, leading to significant economic and public health impacts. Traditional diagnostic methods, mainly serological, often fail to detect seronegative carriers, which continue to spread the infection.

Photocatalytic Degradation of Methylene Blue Dye using PANI-CuFeO Nano Composite.

The present perspective accentuates the synthesis of PANI-CuFeO (PCF) nanocomposite, and photocatalytic degradation of methylene blue (MB) dye using a synthesized composite. The stable PCF is confirmed and characterized by analytical techniques, namely, fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy, X-ray diffraction (XRD), energy-dispersive X-ray (EDX), scanning electron microscopy (SEM), and vibrating sample magnetometry (VSM) analysis. The synthesized PCF nanocomposites are significantly crystalline in nature, having magnetic saturation of 10.

Comparative analysis between multi-strain probiotics and antibiotic as starter feed supplement of poultry on growth performance, serum metabolites and meat quality.

The unobstructed use of antibiotics in poultry production has emerged as a major driving force of antibiotic resistance and public health hazard, particularly in developing countries. This study aimed to determine the functional roles of lyophilized native probiotic based starter feed on performance, selective serum metabolites and meat quality of poultry. A total of 90 day-old birds (30 broilers, 30 layers and 30 ducks) were used as experimental birds which were divided into three treatment groups for each kind of bird.

Multi-omics data integration and drug screening of AML cancer using Generative Adversarial Network.

In the era of precision medicine, accurate disease phenotype prediction for heterogeneous diseases, such as cancer, is emerging due to advanced technologies that link genotypes and phenotypes. However, it is difficult to integrate different types of biological data because they are so varied. In this study, we focused on predicting the traits of a blood cancer called Acute Myeloid Leukemia (AML) by combining different kinds of biological data.

Assessing the Influence of Banana Leaf Ash as Pozzolanic Material for the Production of Green Concrete: A Mechanical and Microstructural Evaluation.

This paper reports an investigation of the mechanical and microscopic properties of partially replaced banana leaf ash (BLA) concrete. In this research, the cement was partially replaced by BLA in two phases: Phase A (0%, 5%, 10%, 15%, 20%, 25% and 30%) and Phase B (0%, 10%, 20% and 30%). The consequence of partially replacing cement with BLA in concrete was investigated by the application of a range of tests, namely X-ray fluorescence (XRF), compressive strength, splitting tensile strength, flexure strength, ultrasonic pulse velocity and scanning electron microscopy (SEM) analysis.

Evaluating arsenic contamination in northwestern Bangladesh: A GIS-Based assessment of groundwater vulnerability and human health impacts.

One of the biggest environmental worries in the world today is the risk of arsenic (As) contamination in groundwater. The Atomic Absorption Spectrometer (AAS) was used in this work to assess the As content in groundwater samples from 38 shallow (27 m) tubewells in northwest Bangladesh to determine the existing situation, potential source(s), and likely health risk of As and other important water quality parameters. The range of arsenic concentrations (μgL) was troublesome and greater than the WHO recommended level for drinking water, ranging from 0.

Stem Extract Facilitated the Synthesis of Iron Oxide Nanoparticles as an Adsorbent to Remove Congo Red Dye.

In this study, a straightforward, eco-friendly, and facile method for synthesizing iron oxide nanoparticles (IONPs) utilizing steam extract as a reducing and stabilizing agent has been demonstrated. The formation of stable IONPs coated with organic moieties was confirmed from UV-vis, FTIR, and EDX spectroscopy and DLS analysis. The produced IONPs are sufficiently crystalline to be superparamagnetic having a saturation magnetization value of 58 emu/g, and their spherical form and size of 9 nm were verified by XRD, VSM, SEM, and TEM investigations.

Unconventionally fast transport through sliding dynamics of rodlike particles in macromolecular networks.

Transport of rodlike particles in confinement environments of macromolecular networks plays crucial roles in many important biological processes and technological applications. The relevant understanding has been limited to thin rods with diameter much smaller than network mesh size, although the opposite case, of which the dynamical behaviors and underlying physical mechanisms remain unclear, is ubiquitous. Here, we solve this issue by combining experiments, simulations and theory.

Green Coffee Bean Extract Assisted Facile Synthesis of Reduced Graphene Oxide and Its Dye Removal Activity.

To discharge the colored effluents from industries there needs to be effective and affordable treatment options. Adsorption using reduced graphene oxide (rGO) as an adsorbent is a prominent one. In this study, green coffee bean extract (GCBE) is utilized as a safe reducing agent for the reduction of graphene oxide (GO) to synthesize rGO.

Green Synthesized Silver Nanoparticles: A Potential Antibacterial Agent, Antioxidant, and Colorimetric Nanoprobe for the Detection of Hg Ions.

Silver nanoparticles (AgNPs) prepared by green synthesis have a lot of potentials in various fields. Among them, as an antioxidant, antibacterial agent, and nanoprobe for the colorimetric detection of mercury (Hg) ions is thought to be the most important. The antibacterial, antioxidant, and colorimetric sensing potential of the greenly produced AgNPs utilizing  stem extract are all predicted in this investigation.

Current Status and De Novo Synthesis of Anti-Tumor Alkaloids in .

Alkaloids are the most diversified nitrogen-containing secondary metabolites, having antioxidant and antimicrobial properties, and are extensively used in pharmaceuticals to treat different types of cancer. Nicotiana serves as a reservoir of anti-cancer alkaloids and is also used as a model plant for the de novo synthesis of various anti-cancer molecules through genetic engineering. Up to 4% of the total dry weight of Nicotiana was found to be composed of alkaloids, where nicotine, nornicotine, anatabine, and anabasine are reported as the dominant alkaloids.

Clinical trials show similar safety outcomes including febrile convulsion rates for GSK's and Merck's measles-mumps-rubella (MMR) vaccines.

Combined measles-mumps-rubella (MMR) vaccines produced by GSK (GSK-MMR) and Merck (Merck-MMR) have demonstrated effectiveness and an acceptable safety profile, as documented over decades of post-licensure use in various regions worldwide. In the United States, 2 doses of the MMR vaccine are recommended at the ages of 12-15 months and 4-6 years. All-cause febrile convulsions have the highest incidence at 12-18 months of age, when the first MMR vaccine dose is administered.

Enhancement of electrocatalytic abilities toward CO reduction by tethering redox-active metal complexes to the active site.

Tethering metal complexes, like [Ru(bpy)Cl] (bpy = 2,2'-bipyridine), which are redox-active at low reduction potentials and have the ability to transfer electrons to another complex, to a [Ni(cyclen)] electrocatalyst enhanced the reduction of CO to CO at low overpotentials. The [Ni(cyclen)] electrocatalyst was modified by tethering redox-active metal complexes 4-methylpyridyl linkers. The redox-active metal complexes were reduced after CO bound to the active site.

Randomised controlled trial showed long-term efficacy, immunogenicity and safety of varicella vaccines in Norwegian and Swedish children.

Aim: Several countries, such as Norway and Sweden, have not implemented universal varicella vaccination. We present data for Norway and Sweden that were generated by a paediatric multi-country Phase III study over a 10-year period. This assessed the efficacy, antibody persistence and safety of two varicella vaccines containing the same Oka strain.

A randomized trial assessing the efficacy, immunogenicity, and safety of vaccination with live attenuated varicella zoster virus-containing vaccines: ten-year follow-up in Russian children.

In Russia, a universal varicella vaccination (UVV) program has not been implemented, and varicella vaccination coverage is low. We assessed the efficacy, antibody persistence, and safety of one- and two-dose varicella vaccination schedules in Russian children with a ten-year follow-up period, as part of an international phase IIIB, observer-blind, randomized, controlled trial (NCT00226499). Children aged 12-22 months were randomized (3:3:1) to receive two doses of tetravalent measles-mumps-rubella-varicella vaccine (V2 group), one dose trivalent measles-mumps-rubella (MMR) vaccine and one dose of varicella vaccine (V1 group), or two doses of MMR vaccine (V0 [control] group), 42 days apart.

Bioinspired in situ repeatable self-recovery of superhydrophobicity by self-reconstructing the hierarchical surface structure.

Inspired by the biological self-recovery mechanism of superhydrophobicity, a new class of waxgel material with sustainable hierarchical surface micro-structures has been reported. After being damaged or removed, the waxgel material can self-reconstruct its surface layer both chemically and structurally, as well as successfully recovers its superhydrophobicity. In addition, it shows non-fluorinated composition, durability to severe mechanical challenges, and self-recoverable surface structures without external input of any kind such as; heat, UV, plasma etc.

Ten-year follow-up on efficacy, immunogenicity and safety of two doses of a combined measles-mumps-rubella-varicella vaccine or one dose of monovalent varicella vaccine: Results from five East European countries.

Background: We assessed the 10-year efficacy, immunogenicity and safety of two doses of a combined measles-mumps-rubella-varicella vaccine (MMRV) or one dose of a monovalent varicella vaccine (V) in children from Czech Republic, Lithuania, Poland, Romania and Slovakia.

Methods: This was a phase IIIB follow-up of an observer-blind, randomized, controlled trial (NCT00226499). In phase A, healthy children aged 12-22 months from 10 European countries were randomized in a 3:3:1 ratio to receive two doses of MMRV (MMRV group), one dose of MMR followed by one dose of V (MMR + V group), or two doses of MMR (MMR; control group), 42 days apart.

Correlation of protection against varicella in a randomized Phase III varicella-containing vaccine efficacy trial in healthy infants.

Background: Varicella vaccination confers high and long-lasting protection against chickenpox and induces robust immune responses, but an absolute correlate of protection (CoP) against varicella has not been established. This study models the relationship between varicella humoral response and protection against varicella.

Methods: This was a post-hoc analysis of data from a Phase IIIb, multicenter, randomized trial (NCT00226499) conducted in ten varicella-endemic European countries.

Formulation and characterization of tamarind (Tamarindus indica L.) seed kernel powder (TKP) as green adhesive for lignocellulosic composite industry.

The objective of this study was to investigate the basic properties of eco-friendly adhesives produced from Tamarindus indica seed kernel powder (TKP) and to use them for fabricating particleboard. Four different types of adhesive were manufactured from TKP having certain percentage of polyvinyl alcohol (PVA), citric acid (CA), glucose, acetic acid, sodium bicarbonate, urea and potassium permanganate. Solid content, Gelling time, FTIR, Thermogravimetric analysis (TGA), viscosity and single lap joint test for shear strength of the prepared adhesives were tested.

Immunogenicity of the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) administered concomitantly with the meningococcal serogroup B (4CMenB) vaccine in infants: A post-hoc analysis in a phase 3b, randomised, controlled trial.

Background: No data are currently available on immunogenicity of higher-valent pneumococcal conjugate vaccines when co-administered with a 4-component meningococcal serogroup B vaccine (4CMenB).

Methods: Post-hoc analysis of pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) immunogenicity when co-administered with 4CMenB (2 + 1 schedule) and/or a CRM-conjugated meningococcal serogroup C vaccine (MenC-CRM) in a trial assessing 4CMenB reduced schedules and co-administration with MenC-CRM (NCT01339923). Infants were randomized to receive 4CMenB and MenC-CRM (Group 1) or MenC-CRM (Group 2) at 3, 5, and 12 months (M) of age.

Enhancement of electrocatalytic abilities for reducing carbon dioxide: functionalization with a redox-active ligand-coordinated metal complex.

A binary system consisting of a ditopic planar pseudo-pincer ligand (qlca = quinoline-2-carbaldehyde (pyridine-2-carbonyl) hydrazone) coordinated to two metal centres affording [{Ru(bpy)2}(μ-qlca)NiCl2]Cl·4H2O·CH3OH (2) (bpy = 2,2'-bipyridine) is reported. The Ni2+ moiety acts as the electrocatalytic active site for CO2 reduction to CO. The turnover frequency (TOF) increased from 0.

Immune response to the hepatitis B antigen in the RTS,S/AS01 malaria vaccine, and co-administration with pneumococcal conjugate and rotavirus vaccines in African children: A randomized controlled trial.

The RTS,S/AS01 malaria vaccine (Mosquirix) reduces the incidence of Plasmodium falciparum malaria and is intended for routine administration to infants in Sub-Saharan Africa. We evaluated the immunogenicity and safety of 10-valent pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV; Synflorix) and human rotavirus vaccine (HRV; Rotarix) when co-administered with RTS,S/AS01 ( www.clinicaltrials.

Immunologic non-inferiority and safety of the investigational pneumococcal non-typeable Haemophilus influenzae protein D-conjugate vaccine (PHiD-CV) 4-dose vial presentation compared to the licensed PHiD-CV 1-dose vial presentation in infants: A phase III randomized study.

Background: To support vaccination programs in developing countries, a 4-dose vial presentation of pneumococcal non-typeable Haemophilus influenzae protein D-conjugate vaccine (PHiD-CV) was developed. This study assessed immunologic non-inferiority and safety of the investigational PHiD-CV 4-dose versus licensed 1-dose vial presentation in infants.

Methods: In this phase III, mono-center, observer-blind study in Bangladesh, 6-10-week-old infants were randomized 1:1 to receive PHiD-CV primary vaccination (at ages 6, 10, 18 weeks) and a booster dose (at age 9 months) with a 4-dose vial (with preservative, 4DV group) or 1-dose vial (preservative-free, 1DV group).