Publications by authors named "Habib Kallel"

Changes in the chemical composition and antioxidant activity of flaxseed hull during maturation were investigated. P129 hull variety was studied at four maturation stages (St1, St2, St3, and St4). Significant variation in proximate composition and flaxseed hull oil characteristics were observed.

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Flaxseed has been used for centuries for oil extraction. In recent years it has attracted considerable interest as a result of studies which attribute potential health benefits to its components. Among the compounds that present biological activity, phenolic compounds are of special interest.

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The accumulation of triacylglycerols during the development of three varieties of peanuts was monitored in two Tunisian cultivated peanut (Trabelsia (AraT) and Chounfakhi (AraC)) and one wild Tunisian peanut (Arbi (AraA)). The presence of TAGs composed of rare fatty acid residues such as hexacosanoic acid (C(23:0)) and heneicosanoic acid (C(21:0)) among the triacylglycerols C(23:0) LL, C(23:0) OO and C(21:0) LL was noted. The major molecular species of triacylglycerol detected in the three peanut varieties were dioleoyl linoleoyl (OOL), 1,2,3-trioleyl (OOO), 1,2-dioleyl-3-palmitoyl (POO), 1,2-dilinoleoyl-3-oleyl (OLL) and 1-oleoyl-2-linoleoyl-3-linolenoyl (OLLn).

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The aim of the present research is to investigate the effect of harvest date on the composition of flaxseed. Samples were collected at regular intervals from 7 to 56 days after flowering (DAF) and analyzed for phospholipid composition, storage protein content and chemical properties. Phospholipid (PL) percentage of the total lipid decreased from 32.

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A high-performance liquid chromatographic method coupled with mass spectrometry was used to characterise the natural phospholipid (PL) classes and molecular species in flaxseed oils. The PL fraction included phosphatidylethanolamine (PE) (27-40%), phosphatidylinositol (PI) (29-32%), phosphatidylcholine (PC) (7-18%), lysophosphatidylcholine (LPC) (8-21%), phosphatidylglycerol (PG) (1-4%) and phosphatidic acid (PA) (1-9%). The distribution of fatty acids was found to differ between phospholipids.

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Seven 4-desmethylsterols, five triterpene alcohols and three 4α-monomethylsterols were identified by GC-MS during the development of wild peanut, which is Arbi (AraA), and cultivars peanut, which are Trabelsia (AraT) and Chounfakhi (AraC). Our results showed that the maximum level of 4-desmethylsterols (881.07 mg/100 g of oil) was reached at 12 days after flowering (DAF) date of peanut plant in AraA, as well as the highest level of triterpene alcohols (31.

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Policosanol (PC) is a mixture of high molecular weight aliphatic primary alcohols. Literature about the contents and compositions of PC derived from peanut varieties is scarce. Total PC composition and content in whole peanut grain samples from three varieties of peanut (two cultivars, AraC and AraT, and a wild one, AraA) were identified using a gas chromatograph system coupled with a mass spectrophotometer.

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The analysis of the total lipid fraction from the Sayali variety of olive oil was accomplished in the present investigation. Glyceridic, unsaponifiable and flavour fractions of the oil were isolated and identified using several analytical methods. Chromatographic techniques have proven to be suitable for these determinations, especially capillary gas chromatography.

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4-Desmethylsterols, the main component of the phytosterol fraction, have been analyzed during the development of Tunisian peanut kernels ( Arachis hypogaea L.), Trabelsia (AraT) and Chounfakhi (AraC), which are monocultivar species, and Arbi (AraA), which is a wild species, by gas chromatography-mass spectrometry. Immature wild peanut (AraA) showed the highest contents of beta-sitosterol (554.

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The evolution of the composition of sterols and squalene during the maturation of the fruit of three cultivars (Achaak, Perlees and Mazetto) almond (Prunus amygdalus Batsh) was investigated. At the same time the evolution of oleic, linoleic and linolenic fatty acids was also studied. The qualitative and quantitative analyses were made by GC-MS and GC-FID.

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A comparative study was performed to determine the free sterols content and composition during the development of three varieties of linseed (H52, O116 and P129). Seed samples were collected at regular intervals from 7 to 60 days after flowering (DAF). Ten compounds were identified: cholesterol, campesterol, brassicasterol, stigmasterol, beta-sitosterol, Delta5-avenasterol, cycloartenol; 24-methylene cycloartanol, obtusifoliol, citrostadienol.

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Changes in hydrocarbon composition were investigated during maturation of three varieties of linseed (H52, O116, and P129) cultivated in Tunisia. The hydrocarbon fraction of the three linseed oil samples was found to contain mainly n-alkanes and squalene. The greatest decrease of these components occurred between 7 and 21 days after flowering (DAF); thereafter, the changes were slight.

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An experimental investigation was carried out on Tunisian olive-fruits of Meski, Sayali and Picholine cultivars. α-Tocopherol and fatty acids (FA) contents were analyzed, during both ripening and processing, according to the Spanish style. The relationship between oil, unsaponifiable and α-tocopherol contents was determined only during ripening.

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The oil content increases during the maturation of seeds (rise of 30%), but decreases at the end of seed maturation. Differences between SDS-PAGE total protein profiles were shown. Polyunsaturated fatty acids contents increase during middle-maturation.

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Purpose: To study Bardet-Biedl syndrome (BBS) in the Tunisian population and determine the presence of triallelism in the eight identified BBS genes.

Methods: DNA samples were collected from 19 consanguineous Tunisian families with BBS. Genome-wide scans were performed with microsatellite markers in 12 families, and two-point linkage analyses were performed.

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