BAMBI Is a Prognostic Biomarker Associated with Macrophage Polarization, Glycolysis, and Lipid Metabolism in Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide. Affected patients have poor prognoses due to high rates of post-surgical recurrence and metastasis. Bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) reportedly contributes to the development and progression of various human cancers.

Determination of Geographical Origin of Southern Shaanxi Congou Black Teas Using Sensory Analysis Combined with Gas Chromatography-Ion Mobility Spectrometry.

Southern Shaanxi is one of China's high-quality congou black tea production areas. However, the differences in geography, cultivation, and management techniques and production processes lead to uneven qualities of southern Shaanxi congou black tea in different production areas. This study used sensory analysis combined with gas chromatography-ion mobility spectrometry (GC-IMS) to determine southern Shaanxi congou black teas' geographical origin and volatile fingerprints to prevent economic losses caused by fraudulent labeling.

Bovine NMRAL2 Protein Blunts Nitric Oxide Production and Inflammatory Response in Infected Bovine Lung Epithelial Cells.

Tuberculosis (TB), primarily caused by () and (), remains the leading cause of death from a single infectious agent globally. Intracellular survival is crucial for their virulence; yet, the underlying mechanisms are not fully understood. This study aimed to demonstrate the significance of a previously unannotated bovine gene ENSBTAG00000011305 in intracellular survival.

Hypocrellin A from an ethnic medicinal fungus protects against NLRP3-driven gout in mice by suppressing inflammasome activation.

Abnormal activation of NLRP3 inflammasome causes the progression of gout, and no small-molecule inhibitor of NLRP3 has been approved yet for clinical use. In this study we established a nigericin-induced inflammasome activation cell model for screening of a natural product library by measuring IL-1β secretion in cell supernatants. Among 432 compounds tested, we found that hypocrellin A (HA), one of the major active components of a traditional ethnic medicinal fungus Hypocrella bambusea in the Northwest Yunnan of China, exhibited the highest inhibition on IL-1β production (IC = 0.

Helicid: A novel Anti-Staphylococcus aureus adjuvant.

Staphylocoagulase (Coa) plays a critical role in the pathogenicity of Staphylococcus aureus (S. aureus). The present study was undertaken to investigate the underlying mechanism which helicid (HEL) suppressed the virulence factor Coa, as well as to assess the synergistic inhibitory effects of HEL in conjunction with antibiotics, thereby establishing the potential of HEL as an antibacterial adjuvant.

Adsorption and Desorption Behavior of Cr(VI) on Two Typical UV-Aged Microplastics in Aqueous Solution.

Microplastics (MPs) are novel pollutants that can adsorb heavy metals in water environments and migrate together as carriers and are prone to aging due to the light in water. However, few reports have been published on the synergistic behavior and effects of these different types of aged MPs on the adsorption and desorption of Cr(VI). Here, two MP types─polyamide (PA) and polylactic acid (PLA)─were aged by UV irradiation, and the adsorption and desorption behaviors of MPs on Cr(VI) were studied.

Evi1 governs Kdm6b-mediated histone demethylation to regulate the Laptm4b-driven mTOR pathway in hematopoietic progenitor cells.

Ecotropic viral integration site 1 (EVI1/MECOM) is frequently upregulated in myeloid malignancies. Here, we present an Evi1-transgenic mouse model with inducible expression in hematopoietic stem/progenitor cells (HSPCs). Upon induction of Evi1 expression, mice displayed anemia, thrombocytopenia, lymphopenia, and erythroid and megakaryocyte dysplasia with a significant expansion of committed myeloid progenitor cells, resembling human myelodysplastic syndrome/myeloproliferative neoplasm-like (MDS/MPN-like) disease.

Endogenous Substances Utilization for Water Self-Purification Amplification Driven by Nonexpendable HO over a Micro-Potential Difference Surface.

Natural self-purification of water is limited by mass transfer processes between inert oxygen (O) and stable pollutants. This process must rely on large energy inputs and resource consumption, which have become a global challenge in the environmental field. Here, we greatly amplify this self-purification effect of natural dissolved oxygen (DO) by nonexpendable HO triggering a DRC catalyst with a micro-potential difference surface.

Wearable Temperature Sensor Enhanced Volatilomics Technique for Swift and Convenient Detection of Latrogenic Botulism.

Accurately assessing potential side effects following botulinum neurotoxin (BoNT) injection remains a formidable challenge. To address this issue, an innovative approach is developed that combines a wearable temperature sensor with a sophisticated volatilomics technique, aimed at facilitating the rapid and convenient prediction of potential physical discomfort related to latrogenic botulism. The investigation identifies five volatile organic compounds (VOCs)-acetone, styrene, ethanol, 2-pentanone, and n-butano-as promising markers indicative of BoNT poisoning.

Suppressed Protein Translation Caused by MSP-8 Deficiency Determines Fungal Multidrug Resistance with Fitness Cost.

Antifungal resistance, particularly the rise of multidrug-resistance strains, poses a significant public health threat. In this study, the study identifies a novel multidrug-resistance gene, msp-8, encoding a helicase, through experimental evolution with Neurospora crassa as a model. Deletion of msp-8 conferred multidrug resistance in N.

Cistanche deserticola polysaccharide alleviates constipation by regulating intestinal barrier function and intestinal microbiota and their metabolites.

Constipation is a clinical condition characterized by reduced intestinal motility, dry and hardened stool, and prolonged retention. Common constipation medicines are less stable, and prolonged use can lead to dependency and side effects. , a well-known Traditional Chinese Medicine, is frequently used to alleviate constipation.

Perineural spread evaluation of cranial nerves in nasopharyngeal carcinoma: superiority and reliability of isovolumetric MR imaging.

Purpose: The purpose of this study was to investigate the advantage of three-dimensional liver acquisition with volume acceleration-flexible (3D LAVA_Flex) for perineural spread (PNS) status of nasopharyngeal carcinoma (NPC) in comparison with two-dimensional magnetic resonance sequences.

Materials And Methods: Sixty pathological proved NPC patients were prospective enrolled. A protocol included T2-weighted imaging with fat suppression (T2WI fs), T1-weighted imaging (T1WI) without and with contrast enhancement (T1WI ce), and 3D LAVA_Flex was applied for the recruited subject.

Dissecting the cellular reprogramming and tumor microenvironment in left- and right-sided Colorectal Cancer by single cell RNA sequencing.

Sidedness and staging are major sources of tumor microenvironment (TME) differences in colorectal cancer (CRC). Subpopulation compositions of stromal cells and immune cells, and interactions between cells collectively constitute the immunosuppressive microenvironment of CRC. In this study, we comprehensively collected single-cell RNA sequencing data from public databases.

Pancreatic β cell interleukin-22 receptor subunit alpha 1 deficiency impairs β cell function in type 2 diabetes via cytochrome b5 reductase 3.

Impaired β cell function is a hallmark of type 2 diabetes (T2D), but the underlying cellular signaling machineries that regulate β cell function remain unknown. Here, we identify that the interleukin-22 receptor subunit alpha 1 (IL-22RA1), known as a co-receptor for IL-22, is downregulated in human and mouse T2D β cells. Mice with β cell Il22ra1 knockout (Il22ra1βKO) exhibit defective insulin secretion and impaired glucose tolerance after being fed a high-fat diet (HFD) or an HFD/low dose of streptozotocin (STZ).

Cluster synchronization of fractional-order two-layer networks and application in image encryption/decryption.

In this paper, a type of fractional-order two-layer network model is constructed, wherein each layer in the network exhibits distinct topology. Subsequently, the cluster synchronization problem of fractional-order two-layer networks is investigated through a two-step approach. The initial step involves the implementation of finite-time cluster synchronization in the first layer by utilizing a fractional-order finite-time convergence lemma.

Efficacy, safety, and somatosensory comparison of pulsed-field ablation and thermal ablation: outcomes from a 2-year follow-up.

Background: Pulsed-field ablation (PFA), as a nonthermal ablative approach for atrial fibrillation, has attracted much attention in recent years. And there are few comparative studies on PFA versus conventional thermal ablation, including radiofrequency ablation (RFA) and cryoballoon ablation (CBA). The efficacy, safety, and somatic sensation of PFA and thermal ablation need to be further compared.

Knowledge and willingness to implement cardiopulmonary resuscitation among the general public: A study in Hubei Province, China.

Background: The incidence of out-of-hospital cardiac arrest (OHCA) in China is high, yet the treatment rate remains low. Research on the current state of the public's awareness of and willingness to provide cardiopulmonary resuscitation (CPR) in Hubei Province is scarcer.

Objectives: The objective of this research was to explore the general public's knowledge, confidence, and willingness to perform CPR in Hubei Province, China.

TSFF-Net: A deep fake video detection model based on two-stream feature domain fusion.

With the advancement of deep forgery techniques, particularly propelled by generative adversarial networks (GANs), identifying deepfake faces has become increasingly challenging. Although existing forgery detection methods can identify tampering details within manipulated images, their effectiveness significantly diminishes in complex scenes, especially in low-quality images subjected to compression. To address this issue, we proposed a novel deep face forgery video detection model named Two-Stream Feature Domain Fusion Network (TSFF-Net).

PWWP domain-containing protein Crf4-3 specifically modulates fungal azole susceptibility by regulating sterol C-14 demethylase ERG11.

The widespread use of azole antifungals in agriculture and clinical settings has led to serious drug resistance. Overexpression of the azole drug target 14α-demethylase ERG11 (CYP51) is the most common fungal resistance mechanism. However, the presence of additional regulatory proteins in the transcriptional response of is not yet fully elucidated.

PHAX enhanced LIN28B-mediated PBX3 mRNA stability to promote esophageal cancer development.

The abnormal expression of PHAX was observed in esophageal cancer, however, its specific function and mechanism remain to be further elucidated. We demonstrated that PHAX, LIN28B, and PBX3 were upregulated in esophageal cancer, while TET2 was downregulated. Elevated PHAX correlated with adverse outcomes among esophageal cancer patients.

Podocyte YAP ablation decreases podocyte adhesion and exacerbates FSGS progression through α3β1 integrin.

Severe proteinuria in focal segmental glomerulosclerosis (FSGS) is closely associated with decreased adhesion, and subsequent loss, of podocytes. Yes-associated protein (YAP) is a key transcriptional coactivator that plays a significant role in maintaining cellular homeostasis. However, its role in podocyte adhesion and its specific mechanism in FSGS progression remain unclear.

MSR1 in lung squamous cell carcinoma: Prognostic and immunological values in pan-cancer and single-cell analyses and a cohort study.

Objective: Lung squamous cell carcinoma (LUSC) constitutes approximately 40% of lung cancer cases and lacks effective treatments, needing new diagnostic and prognostic tools. Macrophage scavenger receptor 1 (MSR1), as a key receptor in macrophages, is essential in tumor immunity. However, its mechanisms in regulating tumor progression and immunity and its prognostic value in LUSC remain unclear.

Bioinspired Iron Porphyrin Covalent Organic Frameworks-Based Nanozymes Sensor Array: Machine Learning-Assisted Identification and Detection of Thiols.

Given the crucial role of thiols in maintaining normal physiological functions, it is essential to establish a high-throughput and sensitive analytical method to identify and quantify various thiols accurately. Inspired by the iron porphyrin active center of natural horseradish peroxidase (HRP), we designed and synthesized two iron porphyrin covalent organic frameworks (Fe-COF-H and Fe-COF-OH) with notable peroxidase-like (POD) activity, capable of catalyzing 3,3',5,5'-tetramethylbenzidine (TMB) into oxidized TMB with three distinct absorption peaks. Based on these, a six-channel nanozyme colorimetric sensor array was constructed, which could map the specific fingerprints of various thiols.

Antiviral signaling of a type III CRISPR-associated deaminase.

Prokaryotes have evolved diverse defense strategies against viral infection, such as foreign nucleic acid degradation by CRISPR-Cas systems and DNA/RNA synthesis inhibition via nucleotide pool depletion. Here, we report an antiviral mechanism of type III CRISPR-Cas-regulated ATP depletion, where ATP is converted into ITP by CRISPR-Cas-associated adenosine deaminase (CAAD) upon activation by either cA or cA, followed by hydrolysis into IMP by Nudix hydrolase, ultimately resulting in cell growth arrest. The cryo-electron microscopy structures of CAAD in its apo and activated forms, together with biochemical evidence, revealed how cA/cA binds to the CARF domain and abrogates CAAD autoinhibition, inducing substantial conformational changes that reshape the structure of CAAD and induce its deaminase activity.