Digital Visits at Intensive Care Units Post-COVID-19: A Mixed-Methods Implementation Evaluation Study.

Due to visiting restrictions at intensive care units during the COVID-19 pandemic, a digital video technology was developed and implemented. This study evaluated the use of digital visits at four intensive care units after COVID-19. Nurses' use of the technology and managerial perspectives on implementation were examined in an explanatory sequential mixed-methods study.

Taking a stand, ready or not: navigating sensitive end-of-life care conversations in patients with end-stage heart failure.

Aims: Patients with heart failure (HF) often experience delayed identification of palliative care needs. While communication with HF patients and their caregivers is increasingly stressed, systematic conversations about end-of-life care wishes remain a gap. This study explores a dyad experience of Advance Care Planning (ACP) conversations in an HF outpatient clinic.

Towards the DSM-6: The intersection of OCD and psychosis. Expert perspectives on insight in the diagnosis of OCD.

In 1994, the DSM-IV added the specifier "with poor insight" to the diagnostic criteria of obsessive-compulsive disorder (OCD). The DSM-5 went one step further and now allows clinicians to diagnose OCD "with absent insight/delusional beliefs," thereby blurring the long-standing distinction between OCD and psychosis. The present study surveyed OCD experts as to their opinions on the insight specifier and the future classification of OCD.

[Body-Focused Repetitive Behavior Disorders: Classification, Diagnosis and Treatment].

Body-focused repetitive behaviors (BFRBs) such as trichotillomania and skin picking are disorders at the interface of psychiatry/psychology, dermatology and dentistry. The disorders can be both either a consequence or a cause of severe somatic disorders. If BFRBs remain undetected and untreated, they tend to become chronic with at times serious somatic complications.

Quo vadis DSM-6? An expert survey on the classification, diagnosis, and differential diagnosis of body-focused repetitive behaviors.

Background: Many conditions we now call body-focused repetitive behaviors (BFRBs) have been subject to research for several decades, most notably trichotillomania and skin picking. However, the American Psychiatric Association did not combine these conditions into a single category, body-focused repetitive behavior disorders (BFRBDs), until the fifth edition of the DSM (2013). Several aspects of the disorder remain uncertain and controversial.

Identifying Palliative Care Needs in Patients With Heart Failure Using Patient Reported Outcomes.

Context: Heart failure (HF) is considered a multifaceted and life-threatening syndrome characterized by high symptom-burden and significant mortality.

Objectives: To describe the symptom-burden in patients with HF and identify their palliative care needs. In this respect, symptom burden related to sex, age and classification of HF using New York Heart Association Functional Classification (NYHA) were analyzed.

Use of a SC5314 PacBio HiFi reads dataset to close gaps in the reference genome assembly, reveal a subtelomeric gene family, and produce accurate phased allelic sequences.

SC5314 is the most-often used strain for molecular manipulation of the species. The SC5314 reference genome sequence is the result of considerable effort from many scientists and has advanced research into fungal biology and pathogenesis. Although the resource is highly developed and presented in a phased diploid format, the sequence includes gaps and does not extend to the telomeres on its eight chromosome pairs.

Prevalence of body-focused repetitive behaviors in a diverse population sample - rates across age, gender, race and education.

Background: Prevalence estimates for body-focused repetitive behaviors (BFRBs) such as trichotillomania differ greatly across studies owing to several confounding factors (e.g. different criteria).

An improved NRRL YB-4239 genome assembly substantiated by its electrophoretic karyotype.

Pacific Biosciences long-read sequencing was used to improve the genome assembly for strain NRRL YB-4239 (ATCC 11503). The new assembly included eight chromosomes that were substantiated by the electrophoretic karyotype. The nuclear genome was 16.

Domain Adaptive and Generalizable Network Architectures and Training Strategies for Semantic Image Segmentation.

Unsupervised domain adaptation (UDA) and domain generalization (DG) enable machine learning models trained on a source domain to perform well on unlabeled or even unseen target domains. As previous UDA&DG semantic segmentation methods are mostly based on outdated networks, we benchmark more recent architectures, reveal the potential of Transformers, and design the DAFormer network tailored for UDA&DG. It is enabled by three training strategies to avoid overfitting to the source domain: While (1) Rare Class Sampling mitigates the bias toward common source domain classes, (2) a Thing-Class ImageNet Feature Distance and (3) a learning rate warmup promote feature transfer from ImageNet pretraining.

Genome sequence of type strain 16-2883 (ATCC TSD-145), an onygenalean fungus associated with freshwater aquatic turtle shell lesions.

is an onygenalean keratinophilic fungus associated with shell and skin lesions in freshwater aquatic turtles. The genome sequence presented here includes five contigs (ranging in size from 2.8 to 9.

Prevalence of chronic obstructive pulmonary disease among 48,061 digestive tract cancer patients in Europe.

Background: Chronic obstructive pulmonary disease (COPD) shares common risk factors with digestive tract malignancies such as esophageal cancer. However, the prevalence and geographic distribution of COPD in patients with gastrointestinal (GI) cancer is only poorly understood.

Methods: We used the IQVIA's Oncology Dynamics (OD) database to identify a total of 48,061 patients with GI cancer (4,229 esophagus, 7,568 stomach, 27,300 colon, and 8,964 rectum cancer) from Germany, France, Italy, Spain and the UK.

Assessing Als3 Peptide-Binding Cavity and Amyloid-Forming Region Contributions to Invasion of Human Oropharyngeal Epithelial Cells.

Although it is widely recognized that disruption of reduces the invasion of germ tubes into mammalian oral epithelial cells, the mechanism of this interaction was unexplored. strains with structurally informed mutations to remove adhesive activity of the peptide-binding cavity (PBC) or aggregative activity mediated by the amyloid-forming region (AFR) were assessed for their ability to invade cultured human oropharyngeal epithelial cells. Initial assays utilized untreated fungal and epithelial cells.

Development and Use of a Monoclonal Antibody Specific for the Cell-Surface Protein Hwp1.

The cell-surface protein Hwp1 functions in adhesion to the host and in biofilm formation. A peptide from the Gln-Pro-rich adhesive domain of Hwp1 was used to raise monoclonal antibody (MAb) 2-E8. MAb 2-E8 specificity for Hwp1 was demonstrated using a isolate and strains that expressed at least one allele.

Development and validation of monoclonal antibodies specific for Candida albicans Als2, Als9-1, and Als9-2.

Fungal agglutinin-like sequence (Als) cell-surface glycoproteins, best characterized in Candida albicans, mediate adhesive and aggregative interactions with host cells, other microbes, and abiotic surfaces. Monoclonal antibodies (MAbs) specific for each C. albicans Als protein are valuable reagents for gaining insight into Als protein localization and function.

Deletion Increases the Proportion of Small Cells in a Culture Population: Hypothesizing a Novel Role for Als1.

Als1 is a large cell-surface glycoprotein most often discussed for its role in mediating ligand-binding and aggregative interactions. Relative to a wild-type control, deletion of produced a strain that showed delayed germ-tube formation and delayed disease progression in a murine model of disseminated candidiasis. Populations of cultured cells had a higher proportion of smaller cells compared to wild-type or reintegrant control cultures.

Candida albicans evades NK cell elimination via binding of Agglutinin-Like Sequence proteins to the checkpoint receptor TIGIT.

Candida albicans is the most common fungal pathogen and a prevalent cause of deadly bloodstream infections. Better understanding of the immune response against it, and the ways by which it evades immunity, are crucial for developing new therapeutics against it. Natural Killer (NK) cells are innate lymphocytes best known for their role against viruses and tumors.

Peering Into Pir Protein Function and Comparative Genomics of the Pir Family.

The fungal cell wall, comprised primarily of protein and polymeric carbohydrate, maintains cell structure, provides protection from the environment, and is an important antifungal drug target. Pir proteins (proteins with internal repeats) are linked to cell wall β-1,3-glucan and are best studied in . Sequential deletion of genes produces strains with increasingly notable cell wall damage.

Using Genomics to Shape the Definition of the Agglutinin-Like Sequence () Family in the Saccharomycetales.

The agglutinin-like sequence () family is studied because of its contribution to cell adhesion, fungal colonization, and polymicrobial biofilm formation. The goal of this work was to derive an accurate census and sequence for genes in pathogenic yeasts and other closely related species, while probing the boundaries of the family within the Order Saccharomycetales. Bioinformatic methods were combined with laboratory experimentation to characterize 47 novel loci from 8 fungal species.

Prevalence of Lung Metastases among 19,321 Metastatic Colorectal Cancer Patients in Eight Countries of Europe and Asia.

Background: Colorectal cancer is one of the most common malignancies in the Western world, and is responsible for about 10% of annual cancer-related deaths. Especially for UICC stage IV, the probability of survival is significantly reduced. Little is known about risk factors for specific metastatic patterns of colorectal cancer that may also influence patients' overall survival.