Publications by authors named "HORWITZ M"
After short periods of labeling with [3H]thymidine, recently completed adenovirus DNA molecules were isolated and cleaved with restriction endonucleases. The strands (heavy and light) of most of the restriction endonuclease fragments were separated. The pattern of labeling clearly shows an asymmetry of radioactivity on the isolated strands of each restriction endonuclease piece.
View Article and Find Full Text PDFAn enterovirus (EVU-16) previously isolated from children with acute infectious lymphocytosis has been further characterized. The EVU-16 virus sediments as a 135S particle in sucrose gradients, has a density of 1-335 g/ml in CsCl, contains 4 polypeptides and has a single stranded RNA genome sedimenting at 35S. These structural features as well as the presence of a virus-related particle, the procapsid, are similar to those of other enteroviruses.
View Article and Find Full Text PDFIn December, 1974, three cases of botulism occurred in a family; two were fatal. The first patient died after a 10-day illness without botulism being suspected. 4 days later, after a 2-day illness, the second patient was diagnosed as having botulism after a cardiorespiratory arrest; she died 3 days later.
View Article and Find Full Text PDFOn Feb. 3, 1975, 196 (57%) of 344 passengers and 1 steward aboard a commercial aircraft contracted a gastrointestinal illness characterised by nausea, vomiting, abdominal cramps, and diarrhoea; 142 passengers and the steward were admitted to hospital. Symptoms developed shortly after a ham and omelette breakfast had been served.
View Article and Find Full Text PDFArticle Synopsis
- Researchers used the left and right halves of adenovirus type 2 and a hybrid adenovirus to investigate where DNA replication starts.
- The findings support multiple models, suggesting that replication could initiate from both ends of the DNA molecules.
- The replication process appears to be bidirectional, meaning it extends in both directions from the starting point.
Adenovirus type 2 DNA synthesis, either in permissive human cells or nonpermissive monkey cells, becomes independent of protein synthesis after the appearance of progeny viral DNA. In the presence of cycloheximide, semiconservative replication and initiation of progeny molecules can occur.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates how human adenovirus fails to replicate in monkey cells and how this issue can be resolved through coinfection with simian virus 40 (SV40).
- It was found that in the absence of SV40, adenovirus capsid proteins are not produced effectively in monkey cells.
- The enhancement of adenovirus replication by SV40 occurs when both viruses infect the same cells, indicating that the replication block happens before the formation of viral particles.
Intracellular degradation of HeLa and adenovirus type 2 DNA induced by camptothecin.
Biochem Biophys Res Commun
November 1971
Postpartum teaching regarding infant care, behavior, and characteristics: a study.
Bull Am Coll Nurse Midwives
November 1971
Article Synopsis
- - Researchers studied the synthesis of adenovirus type 2 DNA in HeLa cells, focusing on the intermediates that form during the process.
- - They found smaller DNA fragments after labeling with radioactive thymidine, but the shorter Okazaki fragments did not dominate the results at any of the measured times.
- - The DNA intermediates were confirmed to be of viral origin, produced during the G(2) phase of the cell cycle, when the host DNA is not actively being synthesized.
The fully dissociated hexon polypeptide of adenovirus type 2 has a molecular weight of 129,000 (+/-10,000) daltons as determined by high-speed sedimentation equilibrium in 6 m guanidine.
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