Technical Strategy of Bone Graft Augmentation in the Anterior Maxilla.

Bone graft augmentation in the anterior maxilla has a high successful rate. However, suture dehiscence and consequent bone graft exposure can compromise and fail this procedure. Therefore, this article presents a new strategy of closure technique to guarantee the bone graft augmentation.

Interferon immunogenicity: technical evaluation of interferon-alpha 2a.

Observations from some studies with interferon-alpha 2a (IFN-alpha 2a) have shown the presence of neutralizing antibodies in a proportion of patients. As a result, an investigation into the production of antibodies to IFN-alpha 2a was undertaken. A number of technical aspects of its production and storage were investigated, including the possibility of an incorrect structure, which could affect the immunogenicity of the IFN-alpha 2a molecule.

[Possibilities of gene-technological production of drugs, especially also the interferons].

For a long time proteins have been used in Medicine as medication. Human Growth Hormone (isolated from Pituitaries of Cadavers) or insuline (isolated from porcine or bovine pancreas) are examples. Genetic engineering has essentially simplified the production of such well known proteins and on the other hand enabled search for and characterization of new human proteins.

Immobilization of beta-galactosidase for application in organic chemistry using a chelating peptide.

The strong interaction of hexa-histidine fusion proteins with metal chelate adsorbents was utilized to immobilize beta-galactosidase with a hexa-histidine peptide at the N-terminus to the Ni(2+)-nitrilotriacetic acid adsorbent. The fusion protein was cloned and expressed in Escherichia coli. The purified soluble fusion protein showed the same specific activity as the purified beta-galactosidase and retained 64 percent of its beta-galactosidase activity when bound to the adsorbent.

[Prognostic and therapeutic significance of steroid receptors in invasive breast cancer].

It is generally accepted that breast cancer with positive steroid receptors has a better prognosis than with negative receptors. The aim of this study was to analyse the influence of the oestrogen (ER) and progesterone (PR) receptors regarding disease-free interval (DFI) and overall (oa) survival of 441 patients operated on in the Department of Gynaecology of the University Hospital of Zürich. Analysis of the subgroups separated according to menopausal status showed the following results: Premenopausal patients (n = 135): Only PR have a significant influence on the oa-survival (p = 0.

Immobilization of monoclonal antibodies for affinity chromatography using a chelating peptide.

A procedure was developed for oriented immobilization of monoclonal antibodies on a solid support. The technique involves the specific oligosaccharide-directed covalent modification of the monoclonal antibody (mAb) with the chelating peptide, Lys-Gly-(His)6, in conjunction with immobilized metal ion affinity chromatography. Chelating peptide-mAb conjugates with a molar ratio of 2.

[Prognosis of local-regional recurrence in breast carcinoma].

992 primary breast cancers were treated at the Gynaecological Department of the University Hospital of Zürich between 1971 and 1988. Local recurrence (LR) has occurred in 131 patients up to now after a median follow-up of 5.1 years.

Timing of breast cancer surgery within the menstrual cycle: influence on lymph-node involvement, receptor status, postoperative metastatic spread and local recurrence.

Prompted by a report of Hrushesky et al. stating that women operated upon for breast cancer during their perimenstrual period showed a higher risk for developing future metastases than women operated upon during their mid-cycle, we examined the patients with breast carcinoma who were treated at the Gynaecological University Hospital Zürich between 1971 and 1988 with respect to the influence of menstrual cycle phase on certain factors. 104 patients underwent perimenstrual surgery, i.

Anantin--a peptide antagonist of the atrial natriuretic factor (ANF). I. Producing organism, fermentation, isolation and biological activity.

Anantin, a peptide binding to the receptor of the atrial natriuretic factor (ANF) was isolated from a strain of Streptomyces coerulescens. The molecule consists of 17 natural L-amino acids which form a peptidic ring system. It has a MW of 1,871.

[Risk factors in breast carcinoma].

A group of 992 breast cancer patients (risk group, R) was compared with a group of 482 patients hospitalized for non oncologic reasons and matched for age and year of hospitalization (comparison group, C). The findings confirm the following factors as risk factors for breast cancer: nulliparity (R 28.8%, C 17.

[Effectiveness of gynecologic preventive examinations--perspectives].

Genital cancer and breast cancer cases were compiled from the data base of the Swiss working group of the Departments of Gynecology and Obstetrics. The total number of 390,000 cases was divided in two three-year periods (1983-1985 and 1986-1988) and then compared. With the exception of cervical cancer cases, which showed a significant increase of Stage 0/CIN III and a significant decrease of the more advanced stages, we noted a more or less unchanged distribution of the other cancer stages in respect of the manifestations.

Adenocarcinoma of the uterine cervix. An analysis of 84 cases between 1970 and 1988.

Of 1284 cases with cervical carcinoma of Stages 0-IV treated at the Gynaecological University Hospital CH-Zurich between 1970 and 1988, 84 (6.5%) were adenocarcinomas; whereas a previous study performed at our clinic between 1950 and 1970 had only revealed an incidence of 3.2%.

[Labor without prenatal care--an unnecessary challenge].

A group of 431 patients who had given birth, but had not attended regular check-ups during their pregnancy, are compared with a cumulative comparative group. The data is from a databank, belonging to the Swiss gynaecological working group. The following results are striking: Very young women, relatively older childbearing women, a noticeably high number of unmarried women and women from the Mediterranean countries.

Large-scale chromatography of recombinant proteins.

When recombinant proteins are expressed in bacterial cells and subsequently grown in fermentation tanks, there remains the problem of recovering the product in pure form. The empirical knowledge gained upon recovery of recombinant proteins indicates that a one-step purification process is very unlikely to succeed. However, combinations of modern techniques, such as immunoaffinity chromatography or immobilized-metal affinity chromatography, with classical techniques, such as ion-exchange chromatography, seem to be suitable for large-scale recovery of recombinant proteins.

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.

A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography. The new resin is a quadridentate chelate former and specially suitable for metal ions with coordination numbers of six, since two valencies remain for the reversible binding of biopolymers. It has been found to chelate Cu2+ and Ni2+ strongly and to be superior to the known iminodiacetic acid adsorbent.

Specificity of the immunoadsorbent used for large-scale recovery of interferon alpha-2a.

After immunoaffinity chromatography, interferon alpha-2a synthesized in bacteria is not homogeneous. Beside oligomers and monomers, a fragment of molecular weight 15,000 was observed. Amino acid analysis and the determination of the amino terminal amino acid sequence of the fragment indicate that this polypeptide represents an interferon alpha-2a molecule lacking the 22 terminal amino acids.

[Obstetric-gynecologic data collection for quality control and responding to health and political status questions. A survey based on 250,000 patients].

Since January 1, 1983 a total of 250,000 patient records have been stored in computer-compatible form in the data base of the Working Group of Swiss Gynecologic Clinics (59 gynecologic-obstetric departments). The information resulting from this mass of data is commented on and analyzed in concise form. Problems of quality control, health care and status-related policies can be addressed and resolved on the basis of up-to-date data which are always available.

Lipstatin, an inhibitor of pancreatic lipase, produced by Streptomyces toxytricini. II. Chemistry and structure elucidation.

The structure of a new pancreatic lipase inhibitor, lipstatin, produced by Streptomyces toxytricini was determined as (2S,3S,5S,7Z,10Z)-5-[(S)-2-formamido-4-methylpentanoyloxy ]-2-hexyl-3- hydroxy-7,10-hexadecadienoic lactone by spectroscopic and chemical methods. Structurally lipstatin is closely related to the known esterase inhibitor esterastin. It contains a N-formyl-L-leucine side chain instead of the N-acetyl-L-asparagine in esterastin.

Lipstatin, an inhibitor of pancreatic lipase, produced by Streptomyces toxytricini. I. Producing organism, fermentation, isolation and biological activity.

Lipstatin, a new and very potent inhibitor of pancreatic lipase (the key enzyme of intestinal fat digestion) was isolated from Streptomyces toxytricini. Lipstatin contains a beta-lactone structure that probably accounts for the irreversible lipase inhibition. The IC50 of lipstatin for pancreatic lipase is 0.

[Double cerclage. Cervix insufficiency in the middle trimester and its treatment].

The treatment of cervical insufficiency with prolapse of the amniotic sac by double cerclage-a procedure that augurs success-is illustrated on the basis of case descriptions.