Epstein-barr virus-induced cap formation in human lymphoblastoid cells.

Redistribution and consequent cap formation of Epstein-Barr virus adsorbed to human lymphoblastoid cells were studied by indirect membrane immunofluorescence carried out at 0 degrees C. When EBV was adsorbed on cells at 0 degrees C, the cell surface fluorescence had a mostly r-ng-like pattern. However, the ring cells could be transformed into cap cells when warmed at 37 degrees C.

Characteristics of cell lines derived from human leukocytes transformed by different strains of Epstein-Barr virus.

Variation of Epstein-Barr virus (EBV) in respect to its effect on the properties of transformed cells was probed. Human umbilical cord leukocytes from six different individuals were transformed in vitro by either B95-8 (B) or QIMR-WIL (Q) strains of EBV and subsequently 12 lymphoblastoid cell lines (six B-derived and six Q-derived lines) were established. The B lines and Q lines were different in the expression of EBV genome i.

Relationship between Epstein-Barr virus-associated nuclear antigen and cell viability in human cell lines.

The relationship between the cell viability and frequency of cell with Epstein-Barr virus (EBV)-associated nuclear antigen was investigated in cultures of 4 EBV-carrier lymphoblastoid cell lines, by means of anti-complement immunofluorescence. The percentages of viable cells and those of EB nuclear antigen-positive cells were close to each other in the entire course of cultivation of all cell lines. This leads to a conclusion that absence of EB nuclear antigen in a given cell population should be evaluated in relation to cell viability.

Evaluation of Epstein-Barr virus-associated nuclear antigen with various human cell lines.

Critical evaluation of the anti-complement immunofluorescence (ACIF) test for Epstein-Barr virus-associated nuclear antigen (EBNA) in known EBV-associated cell lines was carried out. The ACIF procedure, in which the cell smears were thoroughly air-dried and fixed with carbon tetrachloride (for 15 min at room termperature), provided reproducible staining results with minimum variations. The fixed smears coulb be stored for at least 5 weeks at minus 80 degrees C without loss of reactivity.

Effect of antilymphocyte serum on reovirus infection of mice.

Reovirus type 3 infection in mice was investigated, with particular reference to the effect of antilymphocyte serum. With virus inoculation, a characteristic acute disease was manifested in the mice younger than 5 days old but not in mice 7 days old or older. However, a single injection of antilymphocyte serum, which was prepared in rabbits immunized with mouse thymocytes, followed by virus inoculation induced the acute disease in mice 7 days old or older.