Angiotensin converting enzyme in cultured endothelial cells and growth medium. Relationships to enzyme from kidney and plasma.

We have previously reported that cultured cells from swine aorta possess angiotensin converting enzyme (peptidyldipeptide hydrolase, EC 3.4.15.

Bidimensional sorting in preschoolers with an instrumental learning task.

74 lower- and middle-class children aged 2 1/2, 3 1/2, and 4 1/2 years who were successful at unidimensional sorting of 2 objects by either color or form were given feedback for correct bidimensional sorting of 3 objects, 2 of which had been used in unidimensional testing. Four of the 10 2 1/2-year-olds and 58 of the 64 3 1/2- and 4 1/2-year-olds reached criterion on the bidimensional sorting task. Neither social class nor performance on a verbal comprehension test (the Peabody Picture Vocabulary Test) was significantly correlated with success on either unidimensional or bidimensional sorting, although social class and test scores were significantly correlated.

Adolescent pregnancy.

This paper reviews the literature of the past seven years concerning adolescent pregnancy. Social and motivational factors leading to pregnancy are discussed and weighed. Obstetric aspects of adolescent pregnancy are reviewed as well as the repercussions of this event.

Enzyme activities in endothelial cells and smooth muscle cells from swine aorta.

In populations of cultured arterial endothelial and smooth muscle cells grown under the same conditions, we have measured the total activity per cell of 10 enzymes commonly used as "markers" for subcellular organelles: NADH: ferricyanide reductase, NADH:cytochrome c reductase (rotenone insensitive). NADPH:cytochrome c reductase, alpha-glucosidase, 5'-nucleotidase, alkaline phosphodiesterase I, cytochrome oxidase, monoamine oxidase, cathepsin D, and N-acetyl-beta-glucosaminidase. Significant differences between the cell types were found for 7 of the 10 enzymes tested.

Adsorption of messenger RNA of Novikoff hepatoma, normal liver, and regenerating liver on complementary DNA-cellulose affinity matrices.

Complementary DNA (cDNA)-oligodeoxythmidylate-celluloses were prepared from cDNA copies of polysomal messenger RNA (mRNA) of Novikoff hepatoma, normal rat liver, and regenerating rat liver. cDNA synthesis with reverse transcriptase was approximately 46% with respect to input mRNA with oligodeoxythymidylate-cellulose primer. The cDNA's of normal liver, regenerating liver, and Novikoff hepatomas were used as affinity matrices for hybridization of different mRNA species.

Demonstration of cryoprecipitable immune complexes in pneumococcal pneumonia.

Cold-insoluble protein complexes (cryoprecipitates) can be found in the serum in a variety of infectious diseases. We studied serum cryoprecipitates isolated from three patients with pneumococcal pneumonia by counterimmunoelectrophoresis (CEP) and immunofluorescent technics for the presence of immune complexes. The cryoprecipitates and supernatant serum were tested for pneumococcal capsular polysaccharide (PCP) by CEP at 37 C and 56 C with the appropriate controls.

Determination of less than a nanomol of cerebrosides by high performance liquid chromatography with gradient elution analysis.

A gradient elution high performance liquid chromatography method with detection at 230 nm for the analysis of perbenzoylated cerebrosides containing hydroxy and nonhydroxy fatty acids is described. The quantitative range of the method is 0.5-10 nmol of cerebrosides.

Synthesis and turnover of cerebrosides and phosphatidylserine of myelin and microsomal fractions of adult and developing rat brain.

The synthesis and turnover of cerebrosides and phospholipids was followed in microsomal and myelin fractions of developing and adult rat brains after an intracerebral injection of [U-14C]serine. The kinetics of incorporation of radioactivity into microsomal and myelin cerebrosides indicate the possibility of a precursor-product relationship between cerebrosides of these membranes. The specific radioactivity of myelin cerebrosides was corrected for the deposition of newly formed cerebrosides in myelin.

Soft-palate pigmentation in lung disease, including cancer.

A study of patients in whom soft-palate pigmentation appeared to be associated with pulmonary disease has been reported. A significant number of these patients had diagnosed or suspected bronchogenic carcinoma. The possibility that this oral finding may be predictive of the presence of lung cancer, or of a high degree of probability of future development of lung cancer, indicates a need for additional investigation.

Electrophoresis of human multiple myeloma and Waldenström's macroglobulinemia sera in sodium dodecyl sulfate polyacrylamide gels.

Sera from normal persons and patients with IgA, IgD, IgG, and IgM monoclonal gammopathies were electrophoresed in polyacrylamide gels containing sodium dodecyl sulfate. The gels were stained with Coomassie blue or were used for immunodiffusion. By this method IgG multiple myeloma and IgM Waldenström's macroglobulinemia sera were readily distinguished by electrophoresis alone, whereas IgA and IgD myeloma sera were distinguished by further immunodiffusion against anti-alpha-chain antibody and anti-delta-chain antibody.

Characterization of monosomes produced by aflatoxin B1.

A single injection of 1.5 mg aflatoxin B1 per kg body weight produced approx. 70% disaggregation of rat liver polysomes into monosomes within 18 h.