COVID-19 caused by the Omicron variant in lung transplant recipients: a single center case series.

Background: Although coronavirus disease 2019 (COVID-19) is no longer classified as a Public Health Emergency of International Concern by World Health Organization, its global impact persists. Data on its impact in lung transplant recipients (LTRs) from China remain limited. This study aims to share clinical experiences and provide insights into managing LTRs with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

Jugular Vein Evans Blue Injection for Blood-Brain Barrier Assessment Following Hemorrhagic Stroke in a Mouse Model.

The blood-brain barrier (BBB) is a selectively permeable membrane that plays a crucial role in protecting the brain from harmful substances. Its disruption is associated with various central nervous system (CNS) disorders, including hemorrhagic stroke. Evans Blue (EB) dye, a protein-binding tracer, is commonly used to assess BBB permeability.

Postmortem Changes of Desmin, Talin-2, Vinculin, and Integrin β1 in Chicken Breast and Their Effects on Tenderness.

Costameres are essential for maintaining the integrity of muscle fibers, which affects the meat tenderness. To explore the pattern of alteration in costameres after slaughter, this study investigated the distribution of costamere proteins (desmin, talin-2, vinculin, and integrin β1), their impact on tenderness, and the involved enzymes. Western blot analysis showed that talin-2 significantly degraded in postmortem, while integrin β1 significantly increased at 48 h ( < 0.

Synergistic restoration of spinal cord injury through hyaluronic acid conjugated hydrogel-polydopamine nanoparticles combined with human mesenchymal stem cell transplantation.

Spinal cord injury (SCI) is a devastating disease with limited treatment options due to the restricted regenerative capacity of the central nervous system. The accumulation of reactive oxygen species (ROS) and inadequate endogenous neural stem progenitor cells (eNSPCs) in the lesion site exacerbates neurologic deficits and impedes motor function recovery. We have developed a temperature-responsive hyaluronic acid conjugated hydrogel-polydopamine nanoparticles (PDA NPs) combined with human mesenchymal stem cell (hMSCs) transplantation, denoted as H-P-M hydrogel.

The TaCLE24b peptide signaling cascade modulates lateral root development and drought tolerance in wheat.

Plant peptide hormones play an important role in regulating root development and stress responses. Here, we identify a root-derived CLAVATA3/Endosperm surrounding region-related (CLE) peptide TaCLE24b and its leucine-rich repeat receptor-like kinase CLAVATA1 (CLV1) TaCLV1, which together regulate the formation of lateral root primordium and the outgrowth of lateral root in wheat. Additionally, a loss-of-function mutation of TaSG-D1, encoding a Ser/Thr protein kinase glycogen synthase kinase 3 (STKc_GSK3), also enhances the formation of lateral root primordium and the outgrowth of lateral roots in wheat.

ImmunoPET imaging of Trop2 expression in triple-negative breast cancer using [Cu]Cu-NOTA-Trodelvy-F(ab').

Purpose: The Trop2-targeting antibody-drug conjugate (ADC) sacituzumab govitecan (Trodelvy) has demonstrated remarkable efficacy in patients with metastatic triple-negative breast cancer (TNBC). ImmunoPET imaging offers a noninvasive method to visualize the expression and distribution of target antigens in vivo. In this study, we developed F(ab') fragments of Trodelvy for immunoPET imaging to detect Trop2 expression in TNBC models, aiming to achieve a shorter imaging window.

Mesenchymal Stem Cell Exosome and Fibrin Sealant Composite Enhances Rabbit Anterior Cruciate Ligament Repair.

Background: The anterior cruciate ligament (ACL) fails to heal after rupture, leading to joint instability and an increased risk of osteoarthritis. Mesenchymal stem/stromal cell (MSC) exosomes have reported wide-ranging therapeutic efficacy; however, their potential for augmenting ACL repair remains to be investigated.

Purpose: To evaluate the use of MSC exosomes with fibrin sealant on biological augmentation of ACL healing after suture repair and their effects on ACL fibroblast functions.

Bacillus coagulans TBC169 probiotics for intestinal function recovery after gynecological open surgery: a randomized, double-blind, placebo-controlled trial.

Background: Restoration of intestinal function after open surgery remains a significant challenge in gynecological practice. Bacillus coagulans TBC169 probiotics may enhance intestinal motility and recovery.

Aim: This randomized controlled trial aimed to evaluate the efficacy and safety of Bacillus coagulans TBC169 in promoting intestinal function recovery after gynecological open abdominal surgery.

Transcriptomic and metabolomic analysis of recalcitrant phosphorus solubilization mechanisms in .

Introduction: Phosphorus (P) is a crucial growth-limiting nutrient in soil, much of which remains challenging for plants to absorb and use. Unlike chemical phosphate fertilizers, phosphate-solubilizing microorganisms (PSMs) offer a means to address available phosphorus deficiency without causing environmental harm. PSMs possess multiple mechanisms for phosphorus solubilization.

Effects of sperm DNA fragmentation on embryo morphokinetic parameters and laboratory outcomes in women of different ages during intracytoplasmic sperm treatment cycles.

Background: Whether SDF (sperm DNA fragmentation) influences embryo development and the clinical outcomes of assisted reproductive technology (ART) cycles remains controversial. Oocytes derived from women of different ages have varying abilities to repair SDF.

Objective: This study aimed to explore the correlation between SDF and the morphokinetic parameters of embryos during intracytoplasmic sperm injection (ICSI) treatment cycles with consideration of the different ages of female patients.

polysaccharide alleviated intestinal injuries by mediating antioxidant ability and microbiota.

Introduction: is a well-known medicinal herb with proven bioactivities; however, little is known about the effects of its polysaccharide on intestinal injuries in animals induced by lipopolysaccharide (LPS).

Methods: A total of 30 Institute of Cancer Research (ICR) mice were divided into control (CH), induced (MH), and treated (H) groups. Mice in group H were supplemented with 100 mg/kg polysaccharides, while groups C and M were treated with the same amount of normal saline by gavage for 18 days.

Postmortal epithelial changes of donor corneas impair applicability of a refractive ultraviolet femtosecond laser.

This study evaluates the corneal applicability of a refractive ultraviolet femtosecond laser in postmortal human donor eyes and ex vivo porcine eyes. Refractive lenticule extraction and flap creation were attempted in 10 human donor eyes and 80 ex vivo porcine eyes with and without abrasion of the corneal epithelium. The postmortem interval ranged from 6 to 35 h in the human samples and was set to 4, 24, and 48 h for the porcine specimens.

Evaluating the surgical and oncological outcomes of hepatic artery variations in minimally invasive pancreaticoduodenectomy: insights from 2023 data at a high-volume pancreatic center.

Background: Minimally invasive pancreaticoduodenectomy (MIPD) has seen increased adoption due to advancements in surgical techniques and technology. However, the impact of hepatic artery variations (HAV) and clinically relevant HAV (CR-HAV) on MIPD outcomes remains under-investigated. This study aims to explore the differences in surgical and oncological outcomes of MIPD with or without HAV and CR-HAV.

EGCG improve meat quality, restore lipid metabolism disorder and regulate intestinal flora in high-fat fed broilers.

Excessive oil addition can easily result in decreased disease resistance in broilers, a drop in meat quality, and disorders of glucose and lipid metabolism. Epigallocatechin gallate (EGCG) is an important bioactive component of tea and has been shown to have promising effects on the metabolism of nutrients. This study was aimed at investigating the impact of EGCG supplementation through a high-fat diet (HFD) on production performance, meat quality, lipid metabolism and the influence of intestinal flora in broiler chickens.

Effect of total intravenous-based immediate extubation on patient outcomes in adult liver transplantation: A retrospective cohort study.

Background: Although step-down emergence and extubation are universally practiced after liver transplantation (LT), prolonged mechanical ventilation and positive end-expiratory pressure may enhance predisposition to ventilator-associated complications and may be associated with inferior outcomes.

Methods: We screened 339 patients who underwent LT in this retrospective cohort study. After propensity score matching, 35 patients in Group F (total intravenous-based immediate extubation, TIIE) and 107 patients in Group C (balanced anesthesia with step-down extubation) with balanced and comparable pre- and intraoperative profiles were selected for analysis.

A BCL-xL/BCL-2 PROTAC effectively clears senescent cells in the liver and reduces MASH-driven hepatocellular carcinoma in mice.

Accumulation of senescent cells (SnCs) plays a causative role in many age-related diseases and has also been implicated in the pathogenesis and progression of metabolic dysfunction-associated steatotic liver disease (MASLD). Senolytics that can selectively kill SnCs have the potential to be developed as therapeutics for these diseases. Here we report the finding that 753b, a dual BCL-xL/BCL-2 proteolysis-targeting chimera (PROTAC), acts as a potent and liver-tropic senolytic.

Physalis Calyx seu Fructus relieves chicken intestinal damage to heat via improving the antioxidant ability.

Heat-stress-induced oxidative and inflammatory responses were important factors contributing to chicken intestinal damage. The purpose of this study was based on the antioxidant and anti-inflammatory activities of Physalis Calyx seu Fructus (Jin Deng Long, JDL) to investigate its efficacy and mechanism in relieving chicken heat stress damage. Primary chicken embryo duodenum cells and 90 30-day-old specific-pathogen-free chicken were randomly divided into control and JDL groups to establish heat stress models and .

Utilisation of the Innovative [18F]-Labelled Radiotracer [18F]-BIBD-071 Within HR+ Breast Cancer Xenograft Mouse Models.

Aromatase plays a crucial role in the conversion of androgens to oestrogens and is often overexpressed in hormone-dependent tumours, particularly breast cancer. [18F]BIBD-071, which has excellent binding affinity for aromatase and good pharmacokinetics, has potential for the diagnosis and treatment of aromatase-related diseases. The MCF-7 cell line, which is hormone receptor-positive (HR+), was used in the assessment of the novel [18F]-labelled radiotracer [18F]BIBD-071 via positron emission tomography (PET) imaging of an HR+ breast cancer xenograft model.

CO Plasma Treatment To Prepare the Rear Emitter with a Boron-Doped Hydrogenated Amorphous/Nanocrystalline Silicon Stack for a High-Efficiency Silicon Heterojunction Solar Cell.

A rear emitter with a p-type boron-doped hydrogenated amorphous silicon/nanocrystalline silicon [a-Si:H(p)/nc-Si:H(p)] stack was prepared for the silicon heterojunction (SHJ) solar cell to improve its short-circuit current density (). CO plasma treatment (CO PT) was applied to a-Si:H(p) to facilitate the crystallization of the subsequently deposited nc-Si:H(p). To evaluate the effect of the CO PT, two different nc-Si:H(p) layers with low and high crystallinity (χ) were investigated.

Effect of oral potassium supplementation on urinary potassium excretion and its diagnostic value for primary aldosteronism.

Objectives: We aimed to: (1) explore the effect of oral potassium supplementation on urinary potassium excretion, and (2) evaluate the value of urinary potassium-related indicators in distinguishing primary aldosteronism (PA) from non-PA patients.

Design And Methods: A prospective study of 20 patients with hypertension and hypokalemia caused by renal potassium loss between November 2023 and April 2024 was conducted. Demographic features, 24-hour urine collection before and after potassium supplementation were all collected.

Oxycodone for analgesia in children undergoing endoscopic retrograde cholangiopancreatography: a randomized, double-blind, parallel study.

Background: Postoperative visceral pain is a common complication after endoscopic retrograde cholangiopancreatography (ERCP). In this study, we compared the analgesic and anti-inflammatory effects of oxycodone and fentanyl in children undergoing ERCP.

Methods: A single-center, randomized, double-blind study was conducted at a tertiary care hospital affiliated with Shanghai Jiao Tong University.

Intravenous beta-blockers versus amiodarone on in-hospital mortality and safety profile in adult septic patients.

Objective: In the present study, we aimed to compare in-hospital mortality and safety of intravenous beta-blockers and amiodarone in septic patients with new-onset atrial fibrillation (NOAF). The null hypothesis is that there is no significant difference in in-hospital mortality and safety of Beta-blocker (BBs) and amiodarone in treating NOAF in patients with sepsis.

Design: We conducted a retrospective analysis based on the MIMIC-IV database.

Novel technetium-99m-labelled ribociclib isocyanide derivatives for imaging cyclin-dependent kinase 4/6 (CDK4/6) expression in cancer.

Cyclin-dependent kinase 4/6 (CDK4/6) plays a crucial role in cell cycle regulation, is overexpressed in various cancers and is an important target in the development of radiotracers for tumour imaging. Despite the increasing recognition of CDK4/6 inhibitors in cancer therapy, their application is limited by the lack of suitable biomarkers. Herein, we developed a series of technetium-99m-labelled CDK4/6 radiotracers and utilized a linker optimization strategy to reduce their abdominal uptake and enhance their imaging properties.

Effects of replacing soybean meal with cottonseed meal on growth performance, carcass trait, intestinal development and intestinal microbiota of broiler chickens.

This experiment was conducted to evaluate the effects of replacing soybean meal (SBM) with cottonseed meal (CSM) on growth performance, carcass trait, intestinal development and intestinal microbiota of broilers. A total of 792 one-day-old male Ross 308 broiler chickens were randomly allotted to three treatment groups, with 8 replicates per group and 33 birds per replicate. The control birds were administered a diet consisting primarily of corn and soybean meal.

A randomized, crossover trial of one night of oxygen therapy for obstructive sleep apnea in patients with fibrotic interstitial lung disease.

Background And Objective: There is no satisfactory treatment for obstructive sleep apnea (OSA) in patients with interstitial lung disease (ILD) because of poor tolerance of positive airway pressure (PAP) therapy. Supplemental oxygen therapy has been shown to reduce hypoxemia and is well tolerated in patients with ILD. However, little is known about the effect of nocturnal oxygen supplementation (NOS) on OSA in patients with ILD.