Liver transcriptome response to avian pathogenic Escherichia coli infection in broilers with corticosterone treatment.

Avian pathogenic Escherichia coli (APEC) infection has high morbidity and mortality, and multiple stressors encountered during rearing place poultry in a state of stress. However, research on how poultry cope with APEC infection under stress situation is still limited. In this study, we established a broiler stress model by corticosterone (CORT) administration subcutaneously for 7 consecutive days, followed by APEC challenge intramuscularly.

De novo design of self-assembling peptides with antimicrobial activity guided by deep learning.

Bioinspired materials based on self-assembling peptides are promising for tackling various challenges in biomedical engineering. While contemporary data-driven approaches have led to the discovery of self-assembling peptides with various structures and properties, predicting the functionalities of these materials is still challenging. Here we describe the deep learning-guided de novo design of antimicrobial materials based on self-assembling peptides targeting bacterial membranes to address the emerging problem of bacterial drug resistance.

A novel dominant-negative variant of IRF8 in a mother and son: Clinical, phenotypic and biological characteristics.

Background: The few reported patients with pathogenic IRF8 variants have manifested 2 distinct phenotypes: (1) an autosomal recessive severe immunodeficiency with significant neutrophilia and absence of or significant decrease in monocytes and dendritic cells and (2) a dominant-negative form with only a decrease in conventional type 2 dendritic cells (cDC2s) and susceptibility to mycobacterial disease.

Objectives: Genetic testing of a child with persistent EBV viremia identified a novel IRF8 variant: c.1279dupT (p.

Genome-wide profiling of highly similar paralogous genes using HiFi sequencing.

Variant calling is hindered in segmental duplications by sequence homology. We developed Paraphase, a HiFi-based informatics method that resolves highly similar genes by phasing all haplotypes of paralogous genes together. We applied Paraphase to 160 long (>10 kb) segmental duplication regions across the human genome with high (>99%) sequence similarity, encoding 316 genes.

Discovery of the Low-Hemorrhagic Antithrombotic Effect of Montelukast by Targeting FXIa in Mice.

Background: FXIa (coagulation factor XIa) is considered as a promising antithrombotic target with reduced hemorrhagic liabilities. The objective of this study was to identify a small-molecule inhibitor of FXIa as a potential low-hemorrhagic anticoagulant.

Methods: A high-throughput virtual screening was conducted using a drug repurposing library with the catalytic domain of FXIa as the bait.

Comparison of prophylactic endovascular treatments for threatened or impending internal/common carotid artery blowout syndrome.

Background And Purpose: The outcomes of prophylactic endovascular interventions for patients facing threatened or impending carotid blowout syndrome (CBS) involving the internal/common carotid artery (ICA/CCA) have not been extensively elucidated. We aimed to delineate the specific treatment outcomes for this group of patients.

Materials And Methods: We retrospectively enrolled 109 patients with threatened or impending CBS of the ICA/CCA between 2006 and 2023.

Telemedicine-based integrated management of atrial fibrillation in village clinics: a cluster randomized trial.

In rural China, where healthcare relies on village doctors (nonspecialized practitioners who work exclusively in their village clinics), delivering integrated atrial fibrillation (AF) management poses challenges. We developed a telemedicine-based, village doctor-led integrated care model and conducted a cluster randomized clinical trial to assess its efficacy compared to usual care. A total of 30 village clinics were randomly assigned (1:1) to the intervention or control group, with 1,039 village residents aged ≥65 years with AF (44.

Computed tomography radiomics-based combined model for predicting thymoma risk subgroups: a multicenter retrospective study.

Rationale And Objectives: Accurately distinguishing histological subtypes and risk categorization of thymomas is difficult. To differentiate the histologic risk categories of thymomas, we developed a combined radiomics model based on non-enhanced and contrast-enhanced computed tomography (CT) radiomics, clinical, and semantic features.

Materials And Methods: In total, 360 patients with pathologically-confirmed thymomas who underwent CT examinations were retrospectively recruited from three centers.

Introducing an inert-salt into a solvent-reduced catholyte for improving the electrochemical performance.

A high-energy solvent-reduced catholyte containing an inert-salt is prepared by utilizing the common 2,2,6,6-tetra-methylpiperidine-1-oxyl. The novel catholyte can remain liquid at room temperature and achieve a high energy density of 196 W h L by adding a small amount of organic solvent and inert-salt TBAPF. This work provides a significant direction for improving the energy density and stability of RFBs.

Predicting cell properties with AI from 3D imaging flow cytometer data.

Predicting the properties of tissues or organisms from the genomics data is widely accepted by the medical community. Here we ask a question: can we predict the properties of each individual cell? Single-cell genomics does not work because the RNA sequencing process destroys the cell, not allowing us to verify our predictions. To test the hypothesis, we investigate the approach of using AI to analyze single-cell images obtained from a 3D imaging flow cytometer.

De-escalating Dual Antiplatelet Therapy to Ticagrelor Monotherapy in Acute Coronary Syndrome : A Systemic Review and Individual Patient Data Meta-Analysis of Randomized Clinical Trials.

Background: The role of transitioning from short dual antiplatelet therapy (DAPT) to potent P2Y12 inhibitor monotherapy in patients with acute coronary syndrome (ACS) undergoing drug-eluting stent (DES) implantation remains inconclusive.

Purpose: To compare the effects of de-escalating DAPT to ticagrelor monotherapy versus standard DAPT from randomized clinical trials in patients with ACS.

Data Sources: PubMed, EMBASE, Scopus, and ClinicalTrials.

An interpretable machine learning model assists in predicting induction chemotherapy response and survival for locoregionally advanced nasopharyngeal carcinoma using MRI: a multicenter study.

Objectives: To develop and validate an interpretable and generalized machine learning model using MRI for the individualized prediction of induction chemotherapy (ICT) response and survival in locoregionally advanced nasopharyngeal carcinoma (LANPC).

Methods: A total of 1368 patients who underwent MRI examinations before ICT from three hospitals were retrospectively enrolled and divided into training, internal validation, external validation, and cross-field strength validation cohorts. Significant radiomics and clinical features were selected from coarse to fine.

Genome-wide association study of prostate-specific antigen levels in 392,522 men identifies new loci and improves prediction across ancestry groups.

We conducted a multiancestry genome-wide association study of prostate-specific antigen (PSA) levels in 296,754 men (211,342 European ancestry, 58,236 African ancestry, 23,546 Hispanic/Latino and 3,630 Asian ancestry; 96.5% of participants were from the Million Veteran Program). We identified 318 independent genome-wide significant (P ≤ 5 × 10) variants, 184 of which were novel.

Developing a nomogram for risk prediction of the low T3 syndrome.

The objective of this study is to establish a nomogram for predicting low triiodothyronine syndrome (LTS), and clarify the risk factors of LTS. In this study, 109 LTS patients and 331 non-LTS patients were included as the subjects. The general biochemical information of the subjects was collected, and the relevant factors of LTS were evaluated.

Enhanced heterotrophic denitrification in groundwater using pretreated Ginkgo biloba leaves: Optimized carbon utilization and metabolic function diversity.

Ginkgo biloba leaves (Gbl), as abundant agricultural and forestry residues which contains the quercetin that plays an important role in mediating electron transfer, represent a promising heterotrophic denitrification carbon source. Nonetheless, challenges persist due to concerns over nitrate leaching. This study pioneers the application of pretreated Gbl as external carbon sources for heterotrophic denitrification, with a focus on enhancing carbon bioavailability and mitigating nitrate leaching risks.

SARS-CoV-2 Infection Association with Atherosclerotic Plaque Progression at Coronary CT Angiography and Adverse Cardiovascular Events.

Background Patients with acute SARS-CoV-2 infection are reportedly at increased risk for future cardiovascular events; the mechanism underlying this risk remains unclear. Purpose To evaluate the impact of SARS-CoV-2 infection on coronary inflammation and plaques by using coronary CT angiography (CCTA) and the impact on clinical outcomes. Materials and Methods This retrospective analysis of a prospective study included consecutive patients who underwent serial CCTA between September 2018 and October 2023.

Predicting the risk of invasive fungal infections in ICU sepsis population: the AMI risk assessment tool.

Background: Invasive fungal infections (IFI) represent a significant contributor to mortality among sepsis patients in the Intensive Care Unit (ICU). Early diagnosis of IFI is challenging, and currently, there are no predictive tools for identifying sepsis patients who may develop IFI. Our study aims to develop a predictive scoring system to assess the risk of IFI in patients with sepsis admitted to the ICU.

Association of shared decision-making cessation model and adult smoking cessation rate: A prospective cohort study.

Introduction: It remains unclear whether shared decision-making (SDM) can help smoking cessation. This study aims to determine whether the SDM model increases the 24-week point abstinence rate and medication adherence rate for adult smokers.

Methods: This prospective cohort study, conducted between January 2019 and June 2021, enrolled 1268 adult smokers at the outpatient cessation clinic of a national medical center.

Applications of mass spectrometry imaging in botanical research.

Mass spectrometry imaging (MSI) serves as a valuable tool enabling researchers to scrutinize various compounds, peptides, and proteins within a sample, providing detailed insights at both elemental and molecular levels. This innovative technology transforms information obtained from a mass spectrometer- encompassing ionic strength, mass-to-charge ratio, and ionized molecule coordinates-within a defined region into a pixel-based model. Consequently, it reconstructs the spatial distribution of ions, allowing for a comprehensive understanding of molecular landscapes.

Feasibility and accuracy of continuous intraabdominal pressure monitoring with a capsular device in human pilot trial.

Background: Intrabdominal pressure (IAP) is an important parameter. Elevated IAP can reduce visceral perfusion, lead to intraabdominal hypertension, and result in life-threatening abdominal compartment syndrome. While ingestible capsular devices have been used for various abdominal diagnoses, their application in continuous IAP monitoring remains unproven.

Perch Hydrolysates from Upcycling of Perch Side Streams Accelerate Wound Healing by Enhancing Fibroblasts to Secrete Procollagen I, Fibronectin, and Hyaluronan.

Wound healing incurs various challenges, making it an important topic in medicine. Short-chain peptides from fish protein hydrolysates possess wound healing properties that may represent a solution. In this study, perch hydrolysates were produced from perch side steams using a designed commercial complex enzyme via a proprietary pressure extraction technique.

Genomics yields biological and phenotypic insights into bipolar disorder.

Bipolar disorder is a leading contributor to the global burden of disease. Despite high heritability (60-80%), the majority of the underlying genetic determinants remain unknown. We analysed data from participants of European, East Asian, African American and Latino ancestries (n = 158,036 cases with bipolar disorder, 2.

Assessment of intratumor heterogeneity for preoperatively predicting the invasiveness of pulmonary adenocarcinomas manifesting as pure ground-glass nodules.

Background: The increased use of low-dose computed tomography (CT) for lung cancer screening has improved the detection of ground-glass nodules. However, as the clinical utility of CT findings to predict the invasiveness of pure ground-glass nodules (pGGNs) is currently limited, differentiating pGGNs that indicate invasive adenocarcinoma (IAC) from those that represent other histological entities is challenging. We aimed to quantify intratumor heterogeneity of lung adenocarcinomas characterized by pGGNs on CT to assess its efficacy in predicting IACs before surgery.

Location-specific net water uptake and malignant cerebral edema in acute anterior circulation occlusion ischemic stroke.

Background And Purpose: Early identification of malignant cerebral edema (MCE) in patients with acute ischemic stroke is crucial for timely interventions. We aimed to identify regions critically associated with MCE using the Alberta Stroke Program Early Computed Tomography Score (ASPECTS) to evaluate the association between location-specific-net water uptake (NWU) and MCE.

Materials And Methods: This multicentre, retrospective cohort study included patients with acute ischemic stroke following large anterior circulation occlusion.