Network-Based Methods for Deciphering the Oxidizability of Complex Leachate DOM with OH/O via Molecular Signatures.

In landfill leachates containing complex dissolved organic matter (DOM), the link between individual DOM constituents and their inherent oxidizability is unclear. Here, we resolved the molecular signatures of DOM oxidized by OH/O using FT-ICR MS, thereby elucidating their oxidizability and resistance in concentrated leachates. The comprehensive gradual fragmentation of complex leachate DOM was then revealed through a modified machine-learning framework based on 43 key pathways during ozonation.

US-Guided Thermal Ablation for Secondary Hyperparathyroidism: A Prospective Multicenter Study.

Background Interest in microwave ablation (MWA) and radiofrequency ablation (RFA) use for treating secondary hyperparathyroidism (SHPT) is rising; however, ablation outcomes in patients with SHPT are not well characterized. Purpose To assess the response of parathyroid hormone (PTH), calcium, phosphorus, and alkaline phosphatase (ALP) levels to US-guided parathyroid MWA and RFA and the safety of these treatments in participants with SHPT. Materials and Methods This prospective multicenter cohort study, conducted from September 2017 to March 2022, included participants with SHPT.

Switchable Solvent for Separation and Extraction of Lignin from Lignocellulose Biomass: An Investigation of Chemical Structure and Molecular Weight.

Lignin, the most abundant natural aromatic polymer, holds considerable promise for applications in various industries. The primary obstacle to the valorization of lignin into useful materials is its low molecular weight and diminished chemical reactivity, attributable to its intricate structure. This study aimed to treat lignocellulosic biomass using a switchable solvent (DBU-HexOH/HO) derived from the non-nucleophilic superbase 1,8-diazabicyclo [5.

Extended Rhythm Monitoring to Assess for Ventricular Arrhythmias After Transcatheter Pulmonary Valve Replacement With the Harmony Valve.

Background: Varying rates of nonsustained ventricular tachycardia (NSVT) have been reported early after transcatheter pulmonary valve replacement (TPVR) with the Harmony valve, but data regarding rhythm outcomes beyond hospital discharge are limited. This study aims to characterize ventricular arrhythmias after Harmony TPVR from implant through mid-term follow-up.

Methods: Ventricular arrhythmia data from postimplant telemetry and follow-up extended rhythm monitoring (ERM) were analyzed after Harmony TPVR.

Combined Anterior Cruciate Ligament and Anterolateral Ligament Reconstruction Shows Superior Graft Remodeling, Maturation, and Stability Compared With Isolated Anterior Cruciate Ligament Reconstruction.

Purpose: To compare graft remodeling, as measured by magnetic resonance imaging (MRI), and clinical outcomes between patients who underwent isolated anterior cruciate ligament reconstruction (ACLR) versus combined anterior cruciate ligament and anterolateral ligament reconstruction (ACLR + ALLR).

Methods: A retrospective review was conducted on patients who underwent primary ACLR with quadruple hamstring grafts between January 2019 and March 2022, with a minimum follow-up period of 2 years. Patients were categorized into 2 groups on the basis of the addition of ALLR with tibialis anterior allografts: an isolated ACLR group and an ACLR + ALLR group.

High Shear Stress Reduces ERG Causing Endothelial-Mesenchymal Transition and Pulmonary Arterial Hypertension.

Background: Computational modeling indicated that pathological high shear stress (HSS; 100 dyn/cm) is generated in pulmonary arteries (PAs; 100-500 µm) in congenital heart defects causing PA hypertension (PAH) and in idiopathic PAH with occlusive vascular remodeling. Endothelial-to-mesenchymal transition (EndMT) is a feature of PAH. We hypothesize that HSS induces EndMT, contributing to the initiation and progression of PAH.

Unveiling the Centrosymmetric Effect in the Design of Narrowband Fluorescent Emitters: From Single to Double Difluoroboron Cores.

Narrowband fluorescent emitters are receiving significant attention due to the great potential for creating ultrahigh-definition organic light-emitting diode displays (UHD-OLED). Unveiling innovative mechanisms to design new high-performance narrowband fluorescent emitters is a concerted endeavor in both academic and industrial circles. Theoretical calculations reveal that the centrosymmetric dianilido-bipyridine boron difluoride framework (-DAPBF) exhibits significantly reduced structural relaxation compared to previously reported asymmetric structures with monofluoroboron cores, creating new opportunities for the development of narrowband fluorescent emitters.

Advances in biopharmaceutical products for hemophilia.

Hemophilia is caused by the deficiency of clotting factors due to a single genetic abnormality. Replacement therapies have evolved from plasma-derived to recombinant coagulation factor concentrates but continue to have certain limitations. Monoclonal antibodies are clinical prophylactic treatment options unaffected by inhibitors and have better compliance than coagulation factor concentrates for patients with hemophilia.

Hydroxyl groups introducing NMR strategy for structural elucidation of a heptasaccharide isolated from Trillium tschonoskii.

A heptasaccharide was isolated from an active fraction of Trillium tschonoskii using HILIC and high-temperature PGC chromatography methods. UHPLC-Q/TOF-MS analysis gave this oligosaccharide a degree of polymerization (DP) of 7 and MS/MS showed that it has a six-carbon aldehyde glucan structure with the possible chain 1 → 4 connected. The structure was determined by series 1D and 2D NMR in two solvents DO and DMSO‑d.

Intrinsic structure-function connections of carbon-encapsulated nanoscale zero-valent-iron using various pyrolysis atmospheres.

Carbon-encapsulated nanoscale zero-valent-iron (C@Fe) derived from plant-based extracts has been the subject of growing interest due to its environmental friendliness. However, the effects of various pyrolysis atmospheres on the structure-function connections of C@Fe are still unclear. In this study, three pyrolytic atmospheres, namely Air, N, and 5% H/Ar were selected to fabricate X-C@Fe (X represented as A, N, H) for removing 2,4,6-Trichlorophenol (TCP), and the relationships between their structures and functions were demonstrated.

Suite of Biochemical and Cell-Based Assays for the Characterization of Kirsten Rat Sarcoma (KRAS) Inhibitors and Degraders.

KRAS is an important oncogenic driver which is mutated in numerous cancers. Recent advances in the selective targeting of KRAS mutants via small molecule inhibitors and targeted protein degraders have generated an increase in research activity in this area in recent years. As such, there is a need for new assay platforms to profile next generation inhibitors which improve on the potency and selectivity of existing drug candidates, while evading the emergence of resistance.

Comparative Efficacy of Non-contrast vs. Contrast-enhanced CT Radiomics in Predicting Coronary Artery Plaques Among Patients with Low Agatston Scores.

Rationale And Objectives: Patients with a low Agatston score often present with clinical signs and symptoms suggestive of coronary artery disease, despite having minimal calcium deposits. This study aimed to compare the efficacy of low-dose non-contrast cardiac CT with coronary computed tomography angiography (CCTA) in pericoronary adipose tissue (PCAT) radiomics for predicting coronary artery plaques, using CCTA as the reference standard.

Materials And Methods: This retrospective study analyzed 459 patients with suspected coronary artery disease and a coronary artery calcium score < 100 Agatston units, who were treated between June 2021 and December 2023 at a tertiary hospital.

ET-3/ETBR Mediates Na-Activated Immune Signaling and Kidney Lymphatic Dynamics.

Background: Lymphatic collecting vessels in the kidney are critical in clearing interstitial fluid, macromolecules, and infiltrating immune cells. Dysfunction of the lymphatic vessels can disrupt this process and exacerbate injury-associated inflammation in many disease conditions. We previously found that sodium accumulates within the kidney interstitium during proteinuric kidney injury and elevated sodium environments stimulate isolevuglandin production in antigen-presenting cells, stimulating T cells, and modulating inflammatory responses.

Serum GFAP and UCH-L1 Levels for the Assessment of the Absence of Hemorrhagic Transformation: A Multicenter Prospective Cohort Study.

Background: Currently, validated biomarkers for assessing hemorrhagic transformation (HT) after intravenous thrombolysis (IVT) are lacking. We aimed to validate a test combining GFAP (glial fibrillary acidic protein) and UCH-L1 (ubiquitin C-terminal hydrolase-L1) to indicate the absence of HT after IVT.

Methods: We prospectively enrolled consecutive patients with stroke treated with IVT from 16 hospitals.

Molecular composition difference of electron donating moieties between natural organic matter and effluent organic matter probed by chlorine dioxide.

Lignin- and tannin-like phenolic compounds are shown to be the major compositions of electron donating moieties (EDM) of aquatic natural organic matter (NOM). However, little is known about the compositions of EDMs within effluent organic matter (EfOM). In the present study, chlorine dioxide (ClO) was used as a selectively oxidative probe to investigate the difference in the molecular composition of EDM between NOM and EfOM due to its high selectivity towards electron-rich compounds.

Genetic and microenvironmental evolution of colorectal liver metastases under chemotherapy.

Drug resistance limits the efficacy of chemotherapy for colorectal cancer liver metastasis (CRLM). However, the evolution of CRLM during drug treatment remains poorly elucidated. Multi-omics and treatment response data from 115 samples of 49 patients with CRLM undergoing bevacizumab (BVZ)-based chemotherapy show little difference in genomic alterations in 92% of cases, while remarkable differences are observed at the transcriptomic level.

Marine Geo-Polymer Cement Treated with Seawater, Alkaline Activators, Recycled Particles from Paste, and Recycled Particles from Glass.

This study aims to develop the marine geo-polymer cement that was produced with seawater, recycled particles from paste, recycled particles from glass, and alkaline activators, including NaOH or NaO·3.3SiO. The physicochemical properties and strength of MGPC were investigated with a Uniaxial Compression Test, Particle Size Analysis, Energy Dispersive Spectrometer, X-ray Diffraction, and Thermal-field Emission Scanning Electron Microscopy.

The Effect of the Root Bark of (Lycii Radicis Cortex) on Experimental Periodontitis and Alveolar Bone Loss in Sprague-Dawley Rats.

Lycii Radicis Cortex (LRC), the dried root bark of Mill., has traditionally been used as a medicinal herb in East Asia to treat fever and hyperhidrosis. In the present study, we investigated the effects of LRC extract on ligation-induced experimental periodontitis and associated alveolar bone loss in rats.

Disruption of PCSK9 Suppresses Inflammation and Attenuates Abdominal Aortic Aneurysm Formation.

Background: Abdominal aortic aneurysm (AAA) is a chronic vascular inflammatory disease without effective medications. PCSK9 (proprotein convertase subtilisin/kexin 9), a serine protease from the proprotein convertase family, has recently been associated with AAA in human genome-wide association studies. However, its role in AAA is unknown.

Coulomb Explosion Imaging of Complex Molecules Using Highly Charged Ions.

Rapidly stripping off multiple electrons from the target and triggering complete fragmentation with each constituent atom being charged up are ideal prerequisites for Coulomb explosion imaging. Here, we demonstrate that highly charged ion beam with energy in the Bragg peak region is a powerful tool capable of meeting these requirements. Using the 112.

Prediction of the impact of tobacco waste hydrothermal products on compost microbial growth using hyperspectral imaging combined with machine learning.

The insufficient understanding of the impact of hydrothermal products on the growth characteristics of compost microorganisms presents a significant challenge to the broader implementation of hydrothermal coupled composting for tobacco waste. Traditional biochemical detection methods are labor-intensive and time-consuming, highlighting the need for faster and more accurate alternatives. This study investigated the effects of hydrothermal treatment on tobacco straw products and their influence on compost microorganism growth, using hyperspectral imaging (HSI) technology and machine learning algorithms.

Optimizing Propylene/Propane Sieving Separation through Gate-Pressure Control within a Flexible Organic Framework.

The separation of propylene (CH) and propane (CH) is of great significance in the chemical industry, which poses a challenge due to their almost identical kinetic diameters and similar physical properties. In this work, we synthesized an ultramicroporous flexible hydrogen-bonded organic framework (named HOF-FJU-106) by using molecule 2,3,6,7-tetra (4-cyanophenyl) tetrathiafulvalene (TTF-4CN). The formation of the dimer causes the TTF-4CN molecular to bend and weaken π-stacked interactions, coupled with the flexibility of C≡N H-C hydrogen bonds, which leads to reversible conversion between open and closed frameworks through the mutual slip of adjacent layers/columns under activation and stimulation of gas molecules.

Molecular-scale investigation on the photochemical transformation of dissolved organic matter after immobilization by iron minerals with FT-ICR MS.

The interaction between dissolved organic matter (DOM) and iron minerals has a significant effect on its stabilization and preservation in the environment. In this study, iron minerals with different crystal forms (crystalline goethite and amorphous ferrihydrite) were selected to investigate the photochemical transformation process for DOM immobilized on iron minerals under simulated sunlight irradiation at the molecular scale with the help of Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS). The results showed that a total of 7148 molecules were detected in alkaline-extractable sedimentary DOM, of which 38.