Uncovering the electrical synapse proteome in retinal neurons via proximity labeling.

Through decades of research, we have gained a comprehensive understanding of the protein complexes underlying function and regulation of chemical synapses in the nervous system. Despite the identification of key molecules such as ZO-1 or CaMKII, we currently lack a similar level of insight into the electrical synapse proteome. With the advancement of BioID as a tool for proteomics, it has become possible to identify complex interactomes of a given protein of interest by combining enzymatic biotinylation with subsequent streptavidin affinity capture.

Evaluating the Modified American Academy of Pediatrics Screening Algorithm for Critical Congenital Heart Disease.

Objective:  In 2018, an expert panel recommended two key modifications to the most used algorithm for screening neonates for critical congenital heart disease (CCHD). Our aim was to evaluate the outcomes of the modified algorithm compared with those of the original algorithm in a real-world setting.

Study Design:  We compared the performance characteristics of an original CCHD algorithm used to screen term neonates at a large hospital system between October 26, 2018, and October 15, 2020, and the recommended modified algorithm used between October 15, 2020, and June 30, 2022.

Body size modulates the extent of seasonal diet switching by large mammalian herbivores in Yellowstone National Park.

Prevailing theories about animal foraging behaviours and the food webs they occupy offer divergent predictions about whether seasonally limited food availability promotes dietary diversification or specialization. Emphasis on how animals compete for food predominates in work on the foraging ecology of large mammalian herbivores, whereas emphasis on how the diversity of available foods generally constrains dietary opportunity predominates work on entire food webs. Reconciling predictions about what promotes dietary diversification is challenging because species' different body sizes and mobilities modulate how they seek and compete for resources-the mechanistic bases of common predictions may not pertain to all species equally.

Integrated nexus approach to assessing climate change impacts on grassland ecosystem dynamics: A case study of the grasslands in Tanzania.

This study addresses the intricate interplay between climate, vegetation, and livestock dynamics in Tanzania within the Climate-Vegetation-Livestock (CVL) nexus through a quantitative assessment. By examining the temporal and spatial relationships between vegetation indices (NDVI, EVI, NPP) and key climatic variables (Precipitation, Temperature, Evapotranspiration) from 2009 to 2019, and projecting to 2050, this research aims to elucidate vegetation responses to climate change and its subsequent impacts on livestock. To this end, the relationship between the vegetation dynamics indicators (NDVI, NPP) and climate parameters is evaluated to quantify the vegetation response to climate change using statistical models.