Effect of benzodiazepine derivatives on purified blood cholinesterases in vitro.

The in vitro effect of several benzodiazepine derivatives on purified blood cholinesterases were determined. The results were generally in close agreement with previous results obtained on freshly drawn human blood.

Effect of benzodiazepine derivatives on human blood cholinesterase in vitro.

The in vitro effect of ten benzodiazepine derivatives on the cholinesterases of human plasma and red cells was determined. Flurazepam, temazepam, lorazepam, flunitrazepam and diazepam had an inhibitory effect on plasma cholinesterase of 60--90 per cent and, with the exception of lorazepam, an inhibitory effect of 40--50 per cent on red cell cholinesterase. Clonazepam, oxazepam, chlordiazepoxide, nitrazepam and bromazepam had comparatively minor effects on both enzymes

Effect of anti-inflammatory drugs on lipase in vitro.

Mefenamic acid, and to a lesser extent, flufenamic acid have a significant inhibitory effect on lipolysis as determined by the action of mouse pancreas lipase on the dilauric (didodecanoic) acid ester of fluorescein. Ibuprofen, fenoprofen calcium, phenylbutazone, oxyphenbutazone and alclofenac had comparatively little effect.

Effect of oral antidiabetic drugs on lipase activity in vitro.

Phenformin and metformin have a stimulating effect on lipolysis as determined by the action of mouse pancreas lipase on the dilauric (dido-decanoic) acid ester of fluorescein. Tolbutamide and chlorpropamide have an inhibitory effect.