LncRNA LUCAT1 offers protection against human coronary artery endothelial cellular oxidative stress injury through modulating hsa-miR-6776-5p/LRRC25 axis and activating autophagy flux.

Background: Coronary artery disease (CAD) has become a dominant economic and health burden worldwide, and the role of autophagy in CAD requires further clarification. In this study, we comprehensively revealed the association between autophagy flux and CAD from multiple hierarchies. We explored autophagy-associated long noncoding RNA (lncRNA) and the mechanisms underlying oxidative stress-induced human coronary artery endothelial cells (HCAECs) injury.

Near telomere-to-telomere assembly of the Tarim pigeon (Columba livia) genome.

Pigeons serve as important model animals and commercial poultry. The Tarim pigeon, as a breed of Columba livia, is a locally indigenous breed unique to China. While the genome of C.

Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification.

Large multiprotein machines are central to many biological processes. However, stoichiometric determination of protein complex subunits in their native states presents a significant challenge. This study addresses the limitations of current tools in accuracy and precision by introducing concatemer-assisted stoichiometry analysis (CASA).

Health-promoting lifestyles and influencing factors among pregnant women with urinary incontinence: a multicentre cross-sectional study.

Objective: Lifestyle modification is an important part of non-surgical treatment for urinary incontinence (UI) and is strongly recommended. This study aims to explore the status and influencing factors of health-promoting lifestyle among pregnant women with UI.

Design: A multicentre cross-sectional study.

Structural insights into the biochemical mechanism of the E2/E3 hybrid enzyme UBE2O.

The E2/E3 hybrid enzyme UBE2O plays important roles in key biological events, but its autoubiquitination mechanism remains largely unclear. In this study, we determined the crystal structures of full-length (FL) UBE2O from Trametes pubescens (tp) and its ubiquitin-conjugating (UBC) domain. The dimeric FL-tpUBE2O structure revealed interdomain interactions between the conserved regions (CR1-CR2) and UBC.