Long-term nitrogen fertilization alters the partitioning of amino acids between citrus leaves and fruits.

Introduction: The growth of evergreen fruit trees is influenced by the interaction of soil nitrogen (N) and leaf amino acid contents. However, information on free amino acid contents in leaves of fruiting and non-fruiting branches during long-term N fertilizer application remains scarce.

Methods: Here, a four-year field experiment (2018-2021) in a citrus orchard revealed consistently lower total N and amino acid contents in leaves of fruiting compared to non-fruiting branches.

Prognosis after one- and two-stage revision surgery for periprosthetic joint infection: A systematic review and meta-analysis.

Objective: Revision surgeries for periprosthetic joint infections (PJIs) in arthroplasty can follow either one- or two-stage treatment protocols. Previous studies have reported similar reinfection rates and reductions in complication rates for both treatment options. However, the literature on the selection of one protocol is still controversial.

Hidden Threat in Turbid Waters: Quantifying and Modeling the Bioaccumulation and Risks of Particulate Metals to Clams.

A major proportion of metal contaminants in aquatic environments is bound to suspended particulate matter (SPM), yet environmental monitoring typically focuses on dissolved metals, with the filtration step removing SPM. This step may inadvertently hide the potential risks posed by particulate metals. In this study, we used stable isotope tracers to quantify the contributions of SPM-bound metals to the bioaccumulation of nickel (Ni), copper (Cu), zinc (Zn), cadmium (Cd), and lead (Pb) in Ruditapes philippinarum, a widely distributed clam crucial to global aquaculture.

GITRL enhances cytotoxicity and persistence of CAR-T cells in cancer therapy.

CAR T-cell therapy has achieved remarkable clinical success in treating hematological malignancies. However, its clinical efficacy in solid tumors is less satisfactory, partially due to poor in vivo expansion and limited persistence of CAR-T cells. Here, we demonstrated that the overexpression of glucocorticoid-induced tumor necrosis factor receptor-related protein ligand (GITRL) enhances the anti-tumor activity of CAR-T cells.

Technology for the Quantitative Identification of Dairy Products Based on Raman Spectroscopy, Chemometrics, and Machine Learning.

The technologies used for the characterization and quantitative analysis of dairy products based on Raman spectroscopy have developed rapidly in recent years. At the level of spectral data, there are not only traditional Raman spectra but also two-dimensional correlation spectra, which can provide rich compositional and characteristic information about the samples. In terms of spectral preprocessing, there are various methods, such as normalization, wavelet denoising, and feature extraction.