Publications by authors named "H W Rines"
Oat crown rust is one of the most damaging diseases of oat. We identified a new source of resistance and developed KASP and TaqMan markers for selection in breeding programs. A new highly effective resistance to oat crown rust (Puccinia coronata f.
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- 635 oat lines and 4561 SNP loci were analyzed to understand population structure, linkage disequilibrium (LD), and the relationship between genotypes and heading date.
- The analysis revealed that 25.3% of genetic variation could be explained by the first five principal components, but no clear structured population was found, although clustering indicated differences between spring oats and southern U.S. origins.
- Linkage disequilibrium was observed to decay slower in southern oat lines compared to spring oat lines, particularly highlighting an interesting case on linkage group Mrg28, and several linkage groups were consistently associated with heading date across different environments.
Article Synopsis
- The study addresses the challenges of creating a consensus map for the complex hexaploid oat genome (Avena sativa), including the size of the genome and scarcity of molecular markers.
- It introduces new methodologies for discovering SNPs and a novel anchoring strategy, successfully resulting in the first complete physically-anchored consensus map that includes 985 SNPs.
- The findings also highlight genetic similarities with other plants, providing tools for detailed genetic analysis and a useful framework for similar research in other complex genomes.
Hybrid (oat×maize) zygotes developed into euhaploid plants with complete oat chromosome complements without maize chromosomes and into aneuhaploid plants with complete oat chromosome complements and different numbers of retained individual maize chromosomes. The elimination of maize chromosomes in the hybrid embryo is caused by uniparental genome loss during early steps of embryogenesis. Some of these haploid plants set seed in up to 50% of their self-pollinated spikelets.
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- Genetic markers are essential in genomics, but challenges in oat genome complexity and lack of sequence data make finding and testing them difficult; this study aimed to address these issues by generating oat expressed sequence tag (EST) data and developing a method for SNP identification.
- Researchers created a bioinformatics pipeline that processed around 1 million sequence reads, resulting in the identification of 96 in silico SNPs, with 52 found to be polymorphic in a specific oat mapping population, validating their utility through high-resolution melting (HRM) analysis.
- The study concludes that the newly developed high-throughput SNP discovery pipeline and HRM genotyping method are effective for identifying and analyzing genetic diversity in oats, offering a straightforward approach to understanding their complex poly