Detection of antibody to carcinoembryonic antigen by the hemolytic plaque assay.

A modification of the standard hemolytic plaque-forming cell assay is described. The conjugation of a soluble antigen, carcinoembryonic antigen, to the membrane of the sheep red blood cell was accomplished and used as the indicator system to detect antibody-forming cells. The specificity of the assay was determined by cross-reacting studies and competitive inhibition by antigen blocking.

A trial of silver-zinc-allantoinate in the treatment of leg ulcers.

Three hundred thirty-nine of 400 chronic cutaneous ulcers in 264 patients (including some with multiple or bilateral ulcers of both) were healed with silver-zinc-allantoinate creamed (AZAC 1%). Some of the patients treated had failed to respond to medicated wrappings, saline dressings, and various other therapeutic agents, including antibiotics. In one week of treatment with AZAC 1%, bacterial counts were reduced on the average from approximately 2 X 10(7) to 2 X 10(5) (99%).

The use of silver-zinc-allantoin powder for the prehospital treatment of burns.

We describe our experimental studies of a powder formulated to treat serious burn wounds on-the-scene. The wound powder comprises two parts silver-citro-allantoinate, two parts zinc allantoinate and 96 parts pure allantoin. The back skin of 62 rats was shaved and exposed to actively boiling water for ten seconds, resulting in third degree burns of 20% of the total body surface.

Studies on bile salt deconjugation following small bowel bypass procedures.

Using the glycine-l-14C-cholic acid (14C-GCA) test, bile salt deconjugation and excretion were studied in 26 subjects with morbid exogenous obesity before and at selected intervals after jejuno-ileal bypass. In the preoperative group there was no malabsorption or intestinal bacterial deconjugation of the bile salts. In the immediate postoperative period (with relative sterilization of the bowel) there was only a trace of 14C in the breath, but the fecal 14C was highly elevated, indicating severe malabsorption without bacterial activity.

Fat and solid filtration in clinical perfusions.

The purpose of this study was to define the fat filtration characteristics of a packed polyester wool filter when used alone in the cardiotomy suction (12 cases) and the arterial lines (10 cases) during clinical hemodilution, extracorporeal circulation. The pressure gradient-flow relationship and the efficiency of removal of fibrin microparticles were first ascertained in vitro. Total fat was extracted from the polyester wool with a Soxhlet device.