Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.

Three new monoclonal anti-cytokeratin antibodies (mabs) potentially useful in cancer research and clinical diagnosis have been evaluated in immuno-histochemistry on cryostat sections of a broad variety of normal human tissues. A45-B/B3 reacts with all cells containing cytokeratins (epithelia and mesothelia). This mab positively identifies epithelial cells of any kind, and it may serve in differentiating carcinomas from tumours of mesenchymal origin.

[Attempts at production of monoclonal antibodies against human breast tumor cells].

Mouse monoclonal antibodies have been produced by fusion of x 63 Ag 8 . 653 myeloma cells with spleen cells of mice immunized with single cell suspensions of human mammary carcinoma tissue. These antibodies revealed strong differences in binding to cells from malignant and non malignant mammary tissues.

[Monoclonal antibodies against differentiation antigens of carp thymocytes (Cyprinus carpio L.)].

Using the cell fusion technique we have produced monoclonal antibodies directed against carp thymocytes. The monoclonals revealed a different ability for binding to carp lymphocytes of various origin. The antibodies reacting only with lymphocytes seem to react with a membrane structure of a molecular weight of 200 000 to 250 000 daltons.

[Characterization of a series of monoclonal antibodies against human T cells].

A set of 6 monoclonal antibodies is characterized reacting only with human T-cells but not with B lymphocytes. The antibody BL-T1 binds to the E receptor, being there on all mature T cells and on the majority of thymocytes. All respectively the majority of peripheral T cells but only subpopulations of thymocytes are recognized by the monoclonals BL-T2, BL-T3, BL-T4 and BL-T5.