[Characterization and differentiation of fluorescent pseudemonads by substrate utilization studies].

A rapid procedure is described for detecting and differentiating pseudomonads by their ability to degrade substrates with diverse physico-chemical properties, including volatile and non-volatile water-insoluble compounds, strong organic acids and bases, surfactants, disinfectants, and other toxic substances. The bacteria are embedded in an agar medium containing mineral salts and exposed to about 5 mg amounts of six to eight different substrates placed on the surface of an agar plate. After incubation at 25 degrees C for 24 hrs, growth zones around utilizable substrates may be used to differentiate various Pseudomonas strains and species.

[Purification and various properties of NADP+-dependent alcohol dehydrogenase from Acinetobacter calcoaceticus].

The constitutive NADP+-dependent alcohol dehydrogenase from Acinetobacter calcoaceticus can be accumulated about 50 fold in 3 purification steps. The end-product shows in the analytical polyacrylamide gel electrophoresis only one active enzyme band. The molecular weight of the enzyme was determined to be 235,000 by gel chromatography on Sephadex G 200, the smallest subunit shows a molecular weight of 61 000 on SDS electrophoresis.