Optimization of green and rapid analytical procedure for the extraction of patulin in fruit juice and dried fruit samples by air-assisted natural deep eutectic solvent-based solidified homogeneous liquid phase microextraction using experimental design and computational chemistry approach.

In this paper, a green and inexpensive air-assisted natural deep eutectic solvent-based solidified homogeneous liquid phase microextraction procedure was optimized for extraction of patulin in fruit juice and dried fruit samples using experimental design prior to its spectrophotometric determination. Four different natural deep eutectic solvent were prepared and applied to ensure efficient, and selective extraction of patulin. The significant variables including Zn(II) amount, cooling time, pH and amount of natural deep eutectic solvent were optimized by using central composite design.

Metabolic engineering with ATP-citrate lyase and nitrogen source supplementation improves itaconic acid production in .

Background: Bio-based production of organic acids promises to be an attractive alternative for the chemicals industry to substitute petrochemicals as building-block chemicals. In recent years, itaconic acid (IA, methylenesuccinic acid) has been established as a sustainable building-block chemical for the manufacture of various products such as synthetic resins, coatings, and biofuels. The natural IA producer is currently used for industrial IA production; however, the filamentous fungus has been suggested to be a more suitable host for this purpose.

Impact of previous cardiovascular surgery on postoperative morbidity and mortality after major pulmonary resection for non-small cell lung cancer.

Purpose: The aim of this study was to evaluate the impact of previous cardiovascular surgery on the postoperative morbidity and mortality following major pulmonary resection for non-small cell lung cancer (NSCLC).

Methods: Medical records of 227 patients, who underwent major pulmonary resection for NSCLC from 2003 to 2012 at our department, were reviewed retrospectively. Thirty-one patients with a mean age of 65.

Lobar lung resection in elderly patients with non-small cell lung carcinoma: impact of cardiac comorbidity on surgical outcome.

Principles: The aim of this study was to evaluate the impact of cardiac comorbidity on the perioperative morbidity and mortality after lobar lung resection for lung cancer in patients aged 70 years and older.

Methods: The medical records of all 68 patients ≥70 years, who underwent lobar lung resection for non-small cell lung cancer (NSCLC) from 2003 to 2011 at our department, were reviewed retrospectively. Twenty-two patients with a mean age of 76.

Dynamic flux balance analysis for pharmaceutical protein production by Pichia pastoris: human growth hormone.

The influence of methanol feeding rate on intracellular reaction network of recombinant human growth hormone (rhGH) producing Pichia pastoris was investigated at three different specific growth rates, namely, 0.02 (MS-0.02), 0.