Publications by authors named "H Shintaku"
We present a 76-year-old man with cryptogenic new-onset refractory status epilepticus (C-NORSE) with an initial abnormal signal in the nucleus accumbens and a remarkable hyperintense signal on T1-weighted magnetic resonance imaging in the bilateral basal ganglia (BG). His status epilepticus did not respond to most anti-epileptic therapies or immunotherapies, and he died of sepsis. An autopsy revealed severe neuronal loss and hypertrophic astrocytes in the BG and limbic system, with no signs of inflammation or malignancy.
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- Flow cytometry is important in biomedical research but faces challenges with fluctuations in fluorescence intensity, which affects its accuracy.
- A new method has been developed to integrate fluorescence lifetime imaging microscopy (FLIM) into flow cytometry, achieving speeds over 10,000 cells per second.
- This advanced FLIM system can identify subpopulations of cells and observe changes in the nucleus due to anti-cancer drugs, improving the analysis of cellular functions and interactions.
Necroptosis in alveolar epithelial cells drives lung inflammation and injury caused by SARS-CoV-2 infection.
Biochim Biophys Acta Mol Basis Dis
December 2024
COVID-19, caused by SARS-CoV-2 infection, results in irreversible or fatal lung injury. We assumed that necroptosis of virus-infected alveolar epithelial cells (AEC) could promote local inflammation and further lung injury in COVID-19. Since CD8+ lymphocytes induced AEC cell death via cytotoxic molecules such as FAS ligands, we examined the involvement of FAS-mediated cell death in COVID-19 patients and murine COVID-19 model.
View Article and Find Full Text PDFIntroduction: While immune checkpoint inhibitors represent the mainstream treatment for metastatic renal cell carcinoma, a standardized approach following immune checkpoint inhibitors remains unclear. We report a case of metastatic renal cell carcinoma treated with nivolumab rechallenge.
Case Presentation: A 60-year-old male with metastatic melanoma was referred to the urology division due to right renal cancer.
Article Synopsis
- The study introduces ELASTomics, a new method for analyzing both the mechanical properties and genetic expression of individual cells using electroporation to introduce labeled macromolecules into cells for sequencing.
- This technique combines insights into cell surface tension and transcriptional regulation, allowing researchers to investigate how these factors relate to various cell types, including cancer cells and senescent cells.
- ELASTomics can enhance our understanding of cell mechanics and molecular profiles in diverse biological situations, offering a powerful tool for future cellular research.