Publications by H Shimato

Publications by authors named "H Shimato"

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Cloning and expression of the HpaI restriction-modification genes.

H Ito H Shimato A Sadaoka H Kotani F Kimizuka

Nucleic Acids Res

February 1992

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.

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