A novel model of continuous depletion of glutathione in mice treated with L-buthionine (S,R)-sulfoximine.

L-buthionine (S,R)-sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, was administered to mice via drinking water for 14 days in order to establish an animal model with continuously depleted levels of GSH. No toxicity was observed at 20 mM BSO, even though a significant decrease in liver weight was observed at 30 mM BSO. GSH levels in the liver, kidney, brain, lung, heart, spleen, pancreas, small intestine, large intestine, skeletal muscle, plasma and blood cells from mice given 20 mM of BSO were all less than those from the control mice continuously throughout a 24-hr period.

[A case of stimulus-sensitive segmental spinal myoclonus].

A 37-year-old-man was admitted to our hospital because of periodic contraction of the right shoulder muscles of approximately one month's duration. He denied any significant history such as trauma or fever. General physical examination was unremarkable.