Age, sex and gonadal influences on oestrogen receptor content in the rat liver.

Liver cytosol oestrogen receptor (OR) content of intact and gonadectomized male and female rats of defined age (1-18 months) was measured by the controlled pore glass bead assay. The OR content of animals of both sexes aged 3 weeks was low to insignificant (0.12 +/- 0.

Age and incidence of estrogen receptor positive breast tumors.

Estrogen receptors of primary breast cancer tumors were determined by sedimentation analysis. Positive tumors from pre- and post-menopausal patients were those that contained estrogen receptor content greater than 250 and greater than 750 fmoles per g wet weight tissue, respectively. A positive correlation of patients' age with increasing incidence of estrogen receptor positive tumors was found; test for linear trend significance z = 5.

Folate binding protein and the estrogen receptor in breast cancer.

Folate binding protein (FBP) and estrogen receptor (ER) content were determined in primary breast cancers of 48 patients. The mean FBP level was significantly higher in ER-negative tumors than in ER-positive tumors and largely independent of the degree of tumor involvement or menopausal status. FBP correlated negatively with ER and this was most marked for tumors from postmenopausal women.

Post-mortem glycosylated hemoglobin (HbA1c): evidence for a history of diabetes mellitus.

Glycosylated hemoglobin (HbA1c) levels on blood obtained from 28 cadavers were compared with that of healthy volunteers and patients with diabetes mellitus. Levels of HbA1c did not correlate with post-mortem intervals of four to 36 hours, suggesting that HbA1c is stable under these conditions. All deceased with a history of diabetes mellitus, five cases, had an elevated post-mortem blood HbA1c.

Separation of estrogen receptors from nonspecific binding employing receptor antibodies.

Sedimentation analysis in the presence of monoclonal antibodies to the estrogen receptor results in a well-marked separation of the estrogen receptor peak from that containing significant amounts of nonspecific binding. This method has been applied to the study of liver and breast cancer cytosols. These findings are of importance in the study of breast cancer metastases and of endocrine-responsive organs such as the liver, that previously could not be readily analyzed because of the presence of significant amounts of nonspecific binding.