Publications by authors named "H R Wyatt"
Endogenous tagging enables the study of proteins within their native regulatory context, typically using CRISPR to insert tag sequences directly into the gene sequence. Here, we introduce qTAG, a collection of repair cassettes that makes endogenous tagging more accessible. The cassettes support N- and C-terminal tagging with commonly used selectable markers and feature restriction sites for easy modification.
View Article and Find Full Text PDFObjective: While weight status and clinical laboratory measures are important in assessing obesity-related disease severity and chronic disease risk, including a broader range of emotional, psychosocial, and behavioral factors would provide greater context of an individual's overall state of wellness and could be used to better guide treatment decisions. The purpose of this research was to develop a comprehensive Lifestyle Wellness assessment for use in lifestyle-based wellness interventions and programs.
Methods: A cross-sectional exploratory factor analysis (EFA) was conducted using baseline data from = 138 adults participating in behavioral weight loss trials.
Article Synopsis
- The study investigated how cohabitation status affects the success of a web-based weight loss intervention for cancer survivors and their partners, focusing on elements of social cognitive theory like self-efficacy and support.
- Results showed that the intervention positively influenced dietary support and reduced perceived barriers to diet and exercise for non-cohabitating partners, but these effects were not observed among cohabitating couples.
- The findings highlight the importance of social support from partners who do not live together, while also indicating that the dynamics of these relationships need further exploration.
Background: Obesity is commonly aggregated with indices of metabolic health. Proponents of body positivity approaches question whether body size is a determinant of health and well-being. Our objective was to conduct an exploratory factor analysis (EFA) to determine if body size measurements factor load with or independent of metabolic health measures.
View Article and Find Full Text PDFDysregulation of the hematopoietic niche during hyperlipidemia facilitates pathologic leukocyte production, driving atherogenesis. Although definitive hematopoiesis occurs primarily in the bone marrow, during atherosclerosis this also occurs in the spleen. Cells of the bone marrow niche, particularly endothelial cells, have been studied in atherosclerosis, although little is known about how splenic endothelial cells respond to the atherogenic environment.
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