Characterization and quantification of humic substances 2D-Fluorescence by usage of extended size exclusion chromatography.

In this article, two methods for in-depth analysis of humic substances fluorescence are presented. The first one allows the combined analysis of fluorescence excitation-emission matrix (EEM) with chromatography technique. The main issue is the coupling of size exclusion chromatography (SEC) with spectroscopy by the use of an absorption and a fluorescence spectrometer as additional detectors.

Use of cyanopigment determination as an indicator of cyanotoxins in drinking water.

The indicator function of the fluorescence signals of the cyanopigments phycocyanin and phycoerythrin as early warning parameters against the microcystins in drinking water was investigated by lab- and pilot-scale studies. The early warning function of the fluorescence signals was examined with regard to the signals' real-time character, their sensitivity and the behaviour of the cyanopigments in different treatment stages in comparison to microcystins. Fluorescence measurements confirmed the real-time character, since they can be carried out on-site without the pre-concentration of pigments.