Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System.

Protein glycosylation, a critical post-translational modification, influences the stability, efficacy, and immunogenicity of recombinant proteins, including biopharmaceuticals. Glycan structures exhibit significant heterogeneity, varying with production cell types, culture conditions, and purification methods. Consequently, monitoring and evaluating the glycan structures of recombinant proteins is vital, particularly in biopharmaceutical production.

Risk factors of cognitive impairment in pediatric epilepsy patients with focal cortical dysplasia.

Objective: The purpose of this study was to identify the risk factors of cognitive impairment in pediatric epilepsy patients with focal cortical dysplasia (FCD).

Methods: 77 patients with histopathologically confirmed FCD were studied. The statistical relationship between cognition levels and clinical factors at presurgical evaluation was analyzed.

[Single-laboratory validation study of simple and simultaneous determination method for pesticide residues in meat by LC-MS/MS].

We performed a single-laboratory validation study of a simple and simultaneous determination method for pesticide residues in meat using LC-MS/MS. Water was added to the sample and the mixture was homogenized. Next, pesticides were extracted with acetonitrile containing 1 vol% formic acid using a homogenizer, and salted out with magnesium sulfate, trisodium citrate and sodium chloride.

Developmental outcome after surgery in focal cortical dysplasia patients with early-onset epilepsy.

The purpose of this study was to investigate the developmental outcome after surgery for early-onset epilepsy in patients with focal cortical dysplasia (FCD). Among 108 patients with histopathologically confirmed FCD operated between 1985 and 2008, we selected 17 patients with epilepsy onset up to 3 years of age. Development was evaluated by the developmental quotient or intelligence quotient (DQ-IQ) and mental age was measured by the Mother-Child Counseling baby test or the Tanaka-Binet scale of intelligence.

Endoscopic hemostasis with metallic hemoclips for iatrogenic Mallory-Weiss tear caused by endoscopic examination.

Aim: Applied endoscopic techniques including mucosal resection, sclerotherapy and endoscopic retrograde cholangiopancreatography (ERCP) have been advanced and iatrogenic complications including Mallory-Weiss tear (MWT) occasionally occur in daily endoscopic procedures. The present study aimed to examine the advantages of clipping for MWT complications that occur during endoscopic examination.

Methods: Over 10 years, we experienced 47 patients with bleeding caused by MWT.