Publications by authors named "H Nothaft"
Cystic Fibrosis (CF) airway disease is characterized by impaired mucociliary clearance, chronic, polymicrobial infections and robust, neutrophil-dominated inflammation. Pulmonary disease is the leading cause of morbidity and mortality in people with CF and is due to progressive airflow obstruction and ultimately respiratory failure. One of the earliest abnormalities in CF airway disease is the recruitment of neutrophils to the lungs.
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- A live vaccine strain targeting a widespread cause of diarrheal disease in poultry was shown to significantly reduce infection levels in over 50% of vaccinated birds, creating a distinction between "responder" and "non-responder" categories.
- Further studies indicated that the immune response differed between these groups, with responder birds showing stronger antibody activity despite no major overall differences in serum glycosylation patterns.
- Interestingly, the composition of gut microbiota influenced vaccination efficacy, as transferring microbiota from responder birds to non-responders improved immune responses, highlighting the potential for these factors in developing better vaccines for poultry.
is a significant cause of human gastroenteritis worldwide, and all strains express an N-glycan that is added to at least 80 different proteins. We characterized 98 isolates from infants from 7 low- and middle-income countries and identified 4 isolates unreactive with our N-glycan-specific antiserum that was raised against the heptasaccharide composed of GalNAc-GalNAc-GalNAc(Glc)-GalNAc-GalNAc-diNAcBac. Mass spectrometric analyses indicated these isolates express a hexasaccharide lacking the glucose branch.
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- Ecotin, initially found in E. coli, is a strong inhibitor of various serine proteases, including those involved in the immune response, like neutrophil elastase (NE).
- Researchers identified ecotin-like proteins in Campylobacter species linked to periodontal disease and studied their function by expressing them in E. coli and testing their inhibitory effects.
- It was discovered that C. rectus and C. showae ecotins can inhibit NE and support survival in an ecotin-deficient E. coli mutant, suggesting their potential role in protecting against proteases in the oral environment.
is commonly associated with venereal disease and abortions in cattle and sheep, and can also cause intestinal or systemic infections in humans that are immunocompromised, elderly, or exposed to infected livestock. It is also believed that infection can result from the consumption or handling of contaminated food products, but is rarely detected in food since isolation methods are not suited for its detection and the physiology of the organism makes culturing difficult. In the related species, , the ability to colonize the host has been linked to N-linked protein glycosylation with quantitative proteomics demonstrating that glycosylation is interconnected with cell physiology.
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