Effect of a standardized liver and spleen fraction of peptides on the differentiation of human monocyte-derived macrophages.

The effect of Factor AF2 (AF2), a standardized fraction of peptides with a molecular weight of < 10,000 Dalton obtained from livers and spleens of newborn lambs, on the differentiation of human monocyte-derived macrophages was studied, in view of the central role played by these cells in inflammation and tumor cytotoxicity. The results show that the drug 1. increases the cell density of cultures, 2.

Effect of a protein-free dialysate from calf blood on human monocyte differentiation in vitro.

Solcoseryl is a protein-free, standardized dialysate/ultrafiltrate derived from calf blood, which has been shown to improve situations of impaired healing in both experimental animals and man. Its activity seems to be multifactorial although the precise cellular and molecular mechanisms contributing to its effect have not been fully elucidated. Since monocyte-derived macrophages play a central role in inflammation and particularly in wound healing and tissue remodelling, the effect of the dialysate on human monocytes cultured in vitro for 10 days in the presence of human serum was studied.

Potentiation of human polymorphonuclear leukocytes respiratory burst and phagocytosis by a standardized liver and spleen fraction of peptides.

The effect of Factor AF2 (AF2), a xenogeneic fraction of peptides with a molecular weight of < 10,000 Dalton obtained from livers and spleens of newborn lambs, on the oxygen consumption and the phagocytic activity of human polymorphonuclear leukocytes (PMN) was studied. AF2 increased the oxygen uptake of PMN exposed both to serum-treated zymosan (STZ), a phagocytosable stimulus, and phorbol-myristate-acetate (PMA), a soluble stimulus. The potentiating effect of the drug was dose-dependent and more pronounced when suboptimal amounts of either stimulus were used.

Dual effect of a protein-free hemodialysate on the oxygen uptake of phagocytosing human polymorphonuclear leucocytes.

The effect of a protein-free hemodialysate (Solcoseryl) on the oxygen uptake of human polymorphonuclear leucocytes (PMN) was studied. No effect was observed on resting PMN over a concentration range from 0.5 to 10% v/v.