Lysophosphatidylcholines: bioactive lipids generated during storage of blood components.

Transfusion-related acute lung injury (TRALI) is suggested to be a "two hit" event, resulting from priming and activation of pulmonary neutrophils. It is known that neutrophil activation may result from infusion of lysophosphatidylcholines (LysoPCs) accumulated during storage of blood components. The aim of our study was to verify whether the LysoPCs are released into the storage medium of blood components.

The relevance of HPA-15 antigen expression for anti-HPA-15 antibody detection.

Introduction: The HPA-15 antigen system is characterized by a low antigen expression on platelets. The antibodies against this antigen are implied in fetal/neonatal alloimmune thrombocytopenia (F/NAIT), post-transfusion purpura, and refractoriness to platelet transfusions. Detection of these antibodies appears to be related to the level of HPA-15 expression on the platelets used in the monoclonal antibody-specific immobilization of platelet antigen (MAIPA) assay.

Reticulated platelets as a marker of platelet recovery after allogeneic stem cell transplantation.

Reticulated platelets (RP) are the youngest forms of platelets in blood and reflect the rate of bone marrow platelet production. In the present study, we used flow cytometric analysis to determine the percentage of RPs in patients undergoing allogeneic stem cell transplantation. We investigated 10 patients after transplantation from HLA identical siblings: five with acute myeloid leukemia (AML), four with chronic myeloid leukemia (CML), and one patient with myelodysplastic syndrome (MDS).

Transfusion-related acute lung injury and leucocyte-reacting antibodies.

Background And Objectives: Transfusion-related acute lung injury (TRALI) is underdiagnosed and underreported. This is why we present cases suspected for TRALI, in which leucocyte antibodies were examined.

Material And Methods: We analysed 44 patients with respiratory insufficiency, related to transfusion, who met criteria of acute lung injury (ALI).

Leucocyte antibodies in blood donors and a look back on recipients of their blood components.

Background And Aim: The role of leucocyte antibodies in donors is poorly understood in pathogenesis of transfusion-related acute lung injury (TRALI). We examined antibodies in donors and traced recipients transfused with their blood components.

Material And Methods: Antibodies were examined in 1043 donors by five methods, look back performed in 26 recipients.