Immunocytochemical studies of an antigen in a human T-cell lymphoma line (Jurkat) recognized by certain monoclonal antibodies to CD-13 (aminopeptidase-N).

The immunofluorescent staining of Jurkat cells by a panel of eight CD-13 monoclonal antibodies has been investigated. With unfixed cells all antibodies were negative, in contrast to the CD-13-positive cell line, HL60, which gave staining in each case. These results were in line with our previous enzymological studies in which we showed that the aminopeptidase activity observed with intact Jurkat cells was located in the cytoplasm and was distinct in properties from aminopeptidase-N (CD-13), which is expressed by HL60 cells.

Assessment of competency for execution? A guide for mental health professionals.

Assessment of competency for execution presents two compelling ethical questions for mental health professionals: whether clinicians can ethically provide such assessment, and if so, how it should be done in order to maximize quality and minimize ethical conflict. In this article we address the issue of whether to participate and, if so, how. The question of whether to participate is discussed by summarizing the arguments for and against participation and offering guidelines for making a decision.