Automated high-throughput RP-HPLC-MS and SFC-MS analytical and purification platforms to support drug discovery.

In recent years, the need to accelerate drug discovery processes in the pharmaceutical industry has revived the interest of implementing automated workflows, allowing the simultaneous processing of multiple samples on global processes that are referred as High-Throughput Purification (HTP). In this work, SAPIO Laboratory Information Management System (SAPIO LIMS) has been customized at the HTP laboratories of Janssen R&D to accommodate the needs of global purification groups on several automated HTP workflows, integrating Analytical Studio™ data processing tool on multiple steps. Herein we describe the workflow details from crude analysis via RP-LC-MS or SFC-MS systems to sample redissolution and delivery to Compound Logistics (CL) in tubes ready for assay plate preparation.

A Systematic Review on the Impact of Vaccination for Respiratory Disease on Antibody Titer Responses, Health, and Performance in Beef and Dairy Cattle.

Bovine respiratory disease (BRD) is a multifactorial disease complex commonly affecting beef and dairy operations. Vaccination against major BRD-related pathogens is routinely performed for disease prevention; however, uniform reporting of health and performance outcomes is infrequent. Our objective was to evaluate the effect of commercially available BRD-pathogen vaccination on titer response with respect to health or performance in beef and dairy cattle.

An improved method to assess the encapsulation response in arthropods.

Ecoimmunology explores how ecological factors and evolutionary processes influence immune responses across various taxa and how immune responses trade-off with other traits. Studying immune responses requires biologically meaningful immunoassays applicable to a broad range of taxa and are sensitive enough to detect changes in the immune response. Useful immunoassays should also correlate with immunocompetence and fitness.

Understanding the Role of Biofilms in Acute Recurrent Tonsillitis through 3D Bioprinting of a Novel Gelatin-PEGDA Hydrogel.

Acute recurrent tonsillitis is a chronic, biofilm-related infection that is a significant burden to patients and healthcare systems. It is often treated with repeated courses of antibiotics, which contributes to antimicrobial resistance. Studying biofilms is key to understanding this disease.