IgA Serological Response for the Diagnosis of Mycobacterium abscessus Infections in Patients with Cystic Fibrosis.

The immunoglobulin A (IgA) status of cystic fibrosis (CF) patients, presenting with or without a non-tuberculous mycobacterial (NTM) infection, has to date not been fully elucidated toward two antigenic preparations previously described. We have chosen to determine the clinical values of an IgA ELISA for the diagnosis of NTM and/or Mycobacterium abscessus infections in CF patients. One hundred and 73 sera from CF patients, comprising 33 patients with M.

Serological biomarkers for the diagnosis of Mycobacterium abscessus infections in cystic fibrosis patients.

Background: Culture conditions sometimes make it difficult to detect non-tuberculous mycobacteria (NTM), particularly Mycobacterium abscessus, an emerging cystic fibrosis (CF) pathogen. The diagnosis of NTM positive cases not detected by classical culture methods might benefit from the development of a serological assay.

Methods: As part of a diagnostic accuracy study, a total of 173 sera CF-patients, including 33 patients with M.

Stimulation by estradiol-17 beta of thymidine kinase activity in the rat uterus.

The action of estradiol-17 beta (E2) on thymidine kinase (TK) activity was studied in uteri from immature female rats. It was demonstrated that a single injection of E2 highly stimulated the enzyme activity which reached its maximum level 24 h after hormone administration. Physiological amounts of E2 were efficient and changes in TK activity were observed exclusively in uterus and liver.

[Induction, by 17 beta-estradiol, of thymidine kinase activity in uteri of rats].

In uteri from adult female rats, Thymidine Kinase activity varied during ovarian cycle and was maximum at metestrus. Subcutaneous injections of 5, 10 or 25 ng of estradiol-17 beta to immature female rats, resulted in a 3, 5 or 10 fold increase of enzyme activity. Moreover, Thymidine Kinase activity was decreased by injection of medroxyprogesterone alone or associated with estradiol-17 beta.