Publications by authors named "H M Worden"

In September 2020, the Western United States experienced anomalously severe wildfires that resulted in carbon monoxide (CO) emissions almost three times the 2001-2019 average. In this study, we investigate the influence of wildfires on atmospheric carbon monoxide (CO) variability through a comparative analysis of observations from the Measurements of Pollution in the Troposphere (MOPITT), the Infrared Atmospheric Sounding Interferometer (IASI), and the Tropospheric Monitoring Instrument (TROPOMI). Our focus is on the North American domain, aiming to understand the differences among these products.

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Extracellular vesicles (EVs) secreted by human brain cells have great potential as cell-free therapies in various diseases, including stroke. However, because of the significant amount of EVs needed in preclinical and clinical trials, EV application is still challenging. Vertical-Wheel Bioreactors (VWBRs) have designed features that allow for scaling up the generation of human forebrain spheroid EVs under low shear stress.

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Background: While pluripotent stem cell (PSC) therapies move toward clinical and commercial applications at a rapid rate, manufacturing reproducibility and robustness are notable bottlenecks in regulatory approval. Therapeutic applications of PSCs require large cell quantities to be generated under highly robust, well-defined, and economically viable conditions. Small-scale and short-term process optimization, however, is often performed in a linear fashion that does not account for time needed to verify the bioprocess protocols and analysis methods used.

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Extracellular vesicles (EVs) secreted by human-induced pluripotent stem cells (hiPSCs) have great potential as cell-free therapies in various diseases, including prevention of blood-brain barrier senescence and stroke. However, there are still challenges in pre-clinical and clinical use of hiPSC-EVs due to the need for large-scale production of a large quantity. Vertical-Wheel bioreactors (VWBRs) have design features that allow the biomanufacturing of hiPSC-EVs using a scalable aggregate or microcarrier-based culture system under low shear stress.

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Top-down estimates using satellite data provide important information on the sources of air pollutants. We develop a sector-based 4D-Var framework based on the GEOS-Chem adjoint model to address the impacts of co-emissions and chemical interactions on top-down emission estimates. We apply OMI NO, OMI SO, and MOPITT CO observations to estimate NO , SO, and CO emissions in East Asia during 2005-2012.

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