Metabolic Diversity and Aero-Tolerance in Anammox Bacteria from Geochemically Distinct Aquifers.

Anaerobic ammonium oxidation (anammox) is important for converting bioavailable nitrogen into dinitrogen gas, particularly in carbon-poor environments. However, the diversity and prevalence of anammox bacteria in the terrestrial subsurface-a typically oligotrophic environment-are little understood. To determine the distribution and activity of anammox bacteria across a range of aquifer lithologies and physicochemistries, we analyzed 16S rRNA genes and quantified hydrazine synthase genes and transcripts sampled from 59 groundwater wells and metagenomes and metatranscriptomes from an oxic-to-dysoxic subset.

Syntactic categories in the speech of young children: the case of the determiner.

There has been a growing trend in recent years towards the attribution of adult-like syntactic categories to young language-learning children. This is based, at least in part, on studies which claim to have found positive evidence for syntactic phrase structure categories in young children's speech. However, these claims contradict the findings of previous research which suggest that the categories underlying children's early multi-word speech are much more limited in scope.

Tetrahymena conjugation-induced genes: structure and organization in macro- and micronuclei.

The physical organization of eight Tetrahymena genes active during conjugation (meiosis) was examined in the somatic (macro-) and germinal (micro-) nuclei of this organism. Three of these genes make transcripts that are only detected during meiotic prophase. Southern blot analyses indicated that all genes examined were present in both nuclei.

Physical stability of insulin formulations.

Insulin aggregation remains a fundamental obstacle to the long-term application of many insulin infusion systems. We here report the effects of physiologic and nonphysiologic compounds on the aggregation behavior of crystalline zinc insulin (CZI) solutions. Under conditions chosen to simulate the most severe that would be encountered in delivery systems (presence of air, continuous motion, and elevated temperature), both highly purified and regular CZI at 5 U/ml formed turbid gels in 5 days.

Examination of insulin formulations using quasi-elastic light scattering.

A selection of neutral insulin formulations from commercially available vials was examined by quasi-elastic light scattering. In preliminary investigation, significant differences in the particulate nature of insulins from different manufacturers were apparent. Some preparations contained only small diameter species believed to be insulin hexamer while others showed evidence of larger aggregates and microcrystals.