Natural deep eutectic solvents in combination with ultrasonic energy as a green approach for solubilisation of proteins: application to gluten determination by immunoassay.

In this work, a fast and miniaturised procedure based on the use of a natural deep eutectic solvent (NADES) in combination with ultrasound-assisted extraction (UAE) has been proposed for gluten determination by a commercial enzyme-linked immunosorbent assay (ELISA). Fourteen NADESs were prepared by combining two natural primary metabolites and water. Studies on NADES viscosity and gluten solubilisation in NADESs and ethanol-water solutions (for comparison purposes) were carried out.

Gastric cancer in Colombia. II. Case-control epidemiologic study of precursor lesions.

Endoscopic surveys in the Department of Narino (Colombia) showed that natives of areas of very high risks for stomach cancer have a higher prevalence of atrophic gastritis and intestinal metaplasia than do persons born in other parts of Narino. In these surveys, diets were compared between individuals with and without evidence of these suspect precursor lesions of stomach cancer and between individuals born in the areas designated as high and low risk for stomach cancer. The composite evidence from the comparisons indicated lettuce to be negatively associated with stomach cancer and raised the possibility to a positive association for corn.