Publications by authors named "H Le Hir"
Article Synopsis
- The NMD pathway helps cells get rid of faulty messenger RNAs (mRNAs) that could cause problems by stopping too early.
- Researchers studied a protein called Upf1 in yeast and found that it works with another protein called Nmd4 to help this decay process.
- The study showed that important parts of these proteins are similar in different organisms, suggesting that the NMD process is mostly the same across many living things.
Article Synopsis
- Exon junction complexes (EJCs) are crucial for mRNA splicing, primarily known for targeting faulty transcripts with premature stop codons for degradation before the last intron.
- The study reveals that EJCs can form stable complexes with stalled ribosomes and details their assembly and disassembly rates, highlighting that 85% of EJCs disassemble through a faster translation-dependent mechanism.
- Analysis through deep RNA sequencing indicates that EJCs preferentially associate with transcripts related to microtubules and centrosomes, suggesting that their stability and behavior vary based on the specific transcript.
Background: The exon junction complex (EJC) is involved in most steps of the mRNA life cycle, ranging from splicing to nonsense-mediated mRNA decay (NMD). It is assembled by the splicing machinery onto mRNA in a sequence-independent manner. A fundamental open question is whether the EJC is deposited onto all exon‒exon junctions or only on a subset of them.
View Article and Find Full Text PDFN6-methyladenosine (m6A), a widespread destabilizing mark on mRNA, is non-uniformly distributed across the transcriptome, yet the basis for its selective deposition is unknown. Here, we propose that m6A deposition is not selective. Instead, it is exclusion based: m6A consensus motifs are methylated by default, unless they are within a window of ∼100 nt from a splice junction.
View Article and Find Full Text PDFHelicases form a universal family of molecular motors that bind and translocate onto nucleic acids. They are involved in essentially every aspect of nucleic acid metabolism: from DNA replication to RNA decay, and thus ensure a large spectrum of functions in the cell, making their study essential. The development of micromanipulation techniques such as magnetic tweezers for the mechanistic study of these enzymes has provided new insights into their behavior and their regulation that were previously unrevealed by bulk assays.
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