The influence of electrospray ion source design on matrix effects.

This study investigates to which extent the design of electrospray ion sources influences the susceptibility to matrix effects (MEs) in liquid chromatography-tandem mass spectrometry (LC-MS/MS). For this purpose, MEs were measured under comparable conditions (identical sample extracts, identical LC column, same chromatographic method and always positive ion mode) on four LC-MS/MS instrument platforms. The instruments were combined with five electrospray ion sources, viz.

Reduction of matrix effects in liquid chromatography-electrospray ionization-mass spectrometry by dilution of the sample extracts: how much dilution is needed?

In this study, the relationship between matrix concentration and suppression of electrospray ionization (matrix effects) was investigated. Ion suppression of pesticides present in QuEChERS extracts was used as an example. Residue-free extracts of four different commodities, avocado, black tea, orange, and rocket (arugula), were fortified with 39 pesticides each.

Compensation of matrix effects by postcolumn infusion of a monitor substance in multiresidue analysis with LC-MS/MS.

This study systematically compares matrix effects in liquid chromatography (LC) coupled by electrospray ionization (ESI) in the positive mode with tandem mass spectrometry (MS/MS) for 129 pesticides in 20 plant matrixes. In total, 2388 analyte/matrix combinations were evaluated. Permanent postcolumn infusion (PCI) of analyte standards was used to quantify matrix effects over the whole chromatographic run time.