Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery.

INTRODUCTION Lipoplex (cationic liposome-DNA complex) is formed via electrostatic interaction of anionic nucleic acids with cationic liposomes. A thin film of lipids is dried on the bottom of a glass tube and rehydrated in an aqueous solution. The resulting liposome suspension is passed through polycarbonate filters of desired pore size.

Pneumococcal Vaccination in Adults.

Pneumococci remain the most common etiology of community-acquired pneumonia in adults, with significant attendant mortality in the elderly. With the recognition of increasing rates of drug-resistant Streptococcus pneumoniae in recent years, efforts to prevent disease through vaccination have gained greater impetus. The 23-valent pneumococcal vaccine is used widely in the United States and provides effective protection against bacteremic pneumococcal disease, particularly in the immunocompetent host.

Drug-resistant Streptococcus pneumoniae in Community-acquired Pneumonia.

The emergence of Streptococcus pneumoniae isolates resistant to not only penicillin, but to other antipneumococcal agents as well, has major public health implications. Drug-resistant S. pneumoniae are distributed worldwide, and resistance has become increasingly prevalent in the United States within the past decade.

Aspects of Pulmonary Infections After Solid Organ Transplantation.

The increasing number of solid organ transplant (SOT) recipients have high rates of pulmonary infections due to bacterial, fungal, and viral pathogens. These patients have unique sets of factors predisposing to infection. Lung and heart-lung transplants are associated with particularly high infection rates.

Fungal Infections of the Lung.

Fungi, both endemic and opportunistic, continue to be recognized as increasingly frequent pulmonary pathogens. Better appreciation of their epidemiology and clinical course, as well as clarification of the roles of the newer triazoles and lipid formulations of amphotericin B in treatment, have occurred within the past few years. Both endemic and opportunistic fungal pulmonary pathogens are reviewed, with emphasis on recent therapeutic advances.