Image capturing, segmentation and data analysis of shredded refuse streams.

Refuse sorting is an important cornerstone of the recycling industry, but ever-changing refuse compositions and the desire to increase recycling rates still pose many unsolved challenges. The digitalisation of refuse sorting plants promises to overcome these challenges by optimising and automatically adapting the sorting process. This publication describes a system for image capturing, segmentation-based refuse recognition and data analysis of shredded refuse streams.

Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo.

Neuronal activity is accompanied by a net outflow of potassium ions (K) from the intra- to the extracellular space. While extracellular [K] changes during neuronal activity are well characterized, intracellular dynamics have been less well investigated due to lack of respective probes. In the current study we characterized the FRET-based K biosensor lc-LysM GEPII 1.

mitoBK is functionally expressed in murine and human breast cancer cells and potentially contributes to metabolic reprogramming.

Alterations in the function of K channels such as the voltage- and Ca-activated K channel of large conductance (BK) reportedly promote breast cancer (BC) development and progression. Underlying molecular mechanisms remain, however, elusive. Here, we provide electrophysiological evidence for a BK splice variant localized to the inner mitochondrial membrane of murine and human BC cells (mitoBK).

Genetically encoded fluorescent sensor to monitor intracellular arginine methylation.

Arginine methylation (ArgMet), as a post-translational modification, plays crucial roles in RNA processing, transcriptional regulation, signal transduction, DNA repair, apoptosis and liquid-liquid phase separation (LLPS). Since arginine methylation is associated with cancer pathogenesis and progression, protein arginine methyltransferases have gained interest as targets for anti-cancer therapy. Despite considerable process made to elucidate (patho)physiological mechanisms regulated by arginine methylation, there remains a lack of tools to visualize arginine methylation with high spatiotemporal resolution in live cells.