Isolation of a Ca2+ or Mg(2+)-activated ATPase (ecto-ATPase) from bovine brain synaptic membranes.

An ATPase was isolated from synaptosomal plasma membranes derived from bovine cerebral cortex. The protein has an apparent molecular mass of 50 kDa and a pI of 5.3 to 5.

Soluble low-Km 5'-nucleotidase from electric-ray (Torpedo marmorata) electric organ and bovine cerebral cortex is derived from the glycosyl-phosphatidylinositol-anchored ectoenzyme by phospholipase C cleavage.

Soluble and membrane-bound low-Km 5'-nucleotidase was isolated from high-speed supernatants and membrane fractions derived from the electric organ of the electric ray (Torpedo marmorata) or from bovine brain cerebral cortex. Purification of both enzymes included chromatography on concanavalin A-Sepharose and AMP-Sepharose. The contribution to the total of soluble enzyme activity was lower in electric organ (1.

Association of the HNK-1 epitope with 5'-nucleotidase from Torpedo marmorata (electric ray) electric organ.

5'-Nucleotidase isolated from the electric organ of the electric ray (Torpedo marmorata) has a molecular mass of 62 kDa and, on two-dimensional electrophoresis, separates into up to 13 isoforms within a pI range of 5.9-6.7.

[Body-packer syndrome. Psychotropic drug smuggle in the human body--discussion from the legal and medical viewpoint based on own patients].

Body packing is the term used to describe the smuggling of drugs, usually cocaine or heroin, in special containers introduced within the body. Rupture of the containers leads to acute intoxication that often ends fatally. The possibility of drug poisoning should always be considered by the emergency or hospital physician confronted with an unconscious patient.

[The "upside-down stomach"--a diagnostic pitfall].

Upside down stomach is an extreme variant of a paraesophageal hernia. The cardia and pylorus are located next to each other, while the rest of the stomach lies within the thorax. Radiology is mandatory for accurate anatomical documentation, while endoscopy is necessary to identify pathology, primarily bleeding sources.