Publications by authors named "H Klein"
Basic Science and Pathogenesis.
Alzheimers Dement
December 2024
Background: Glial cells exhibit distinct transcriptional responses to β-amyloid pathology in Alzheimer's disease (AD). While sophisticated single-cell based methods have revealed heterogeneous glial subpopulations in the human AD brain, the histological localization of these multicellular responses to AD pathology has not been fully characterized due to the loss of spatial information. Here, we combined spatial transcriptomics (ST) with immunohistochemistry to explore the molecular mechanisms in the neuritic plaque niche.
View Article and Find Full Text PDFBackground: The transfer of mitochondrial DNA into the nuclear genomes of eukaryotes (Numts) has been linked to lifespan in non-human species and recently demonstrated to occur in rare instances from one human generation to the next.
Method: Here we investigated numtogenesis dynamics in humans in two ways. First, we quantified Numts in 1,187 post-mortem brain and blood samples from different individuals.
Background: Interventional trials in Alzheimer's Disease (AD) are increasingly targeting early disease stages. To optimize the likelihood of successful outcomes for these trials, there is an important need for tools capable of identifying individuals prone to exhibit clinical progression throughout the course of the trial. The Expanded Brief Assessment of Cognition (Expanded-BAC) and the Virtual Reality Functional Capacity Assessment Tool - Short List (VRFCAT-SLx) are two computerized tests assessing cognitive and functional abilities, respectively.
View Article and Find Full Text PDFThis report highlights the development of Grace House Akron, Inc. (GHA) a comfort care home focused on caring for terminally ill individuals who are unhoused, isolated, without caregivers, economically disadvantaged and face end of life alone. GHA is a free-standing home that provides safe housing, a nurturing environment, and round-the-clock personal care while local hospice agencies provide medical management.
View Article and Find Full Text PDFArticle Synopsis
- Single-nucleus transcriptomic studies have identified specific glial cell states linked to Alzheimer's disease but lack context from the actual structure of the human neocortex.
- This study used an unbiased analytic strategy to analyze spatially-registered transcriptomic data, finding that certain genes, including metallothioneins, are altered near amyloid plaques.
- Validation through immunofluorescence showed that a reactive astrocyte subtype, Ast.5, is involved in the environment around these plaques, indicating its potential role in the disease process.