Publications by authors named "H Kirchherr"
Article Synopsis
- The activity of the thiopurine S-methyltransferase (TPMT) gene significantly affects how patients metabolize and respond to thiopurine drugs, making TPMT testing essential for personalized treatment.
- A new high-performance liquid chromatography (HPLC) method was developed to measure TPMT activity directly in whole blood, demonstrating similar or improved accuracy compared to the traditional erythrocyte method.
- The whole-blood method showed high specificity in distinguishing TPMT activity levels and is now recommended for both clinical and research purposes, with a defined cutoff for normal versus reduced activity levels.
Exercise is rewarding, and long-distance runners have described a runner's high as a sudden pleasant feeling of euphoria, anxiolysis, sedation, and analgesia. A popular belief has been that endogenous endorphins mediate these beneficial effects. However, running exercise increases blood levels of both β-endorphin (an opioid) and anandamide (an endocannabinoid).
View Article and Find Full Text PDFObjectives: Thiopurine drugs (azathioprine, 6-mercaptopurine) show wide interindividual variability and a narrow therapeutic range thus making therapeutic monitoring of their active metabolite 6-thioguanine nucleotides (6-TGN) desirable. We improved the currently available laborious and complex methodology of therapeutic drug monitoring of 6-TGN and the metabolite 6-methylmercaptopurine (6-MMP) in washed erythrocytes (ery) based on a whole-blood method.
Methods: The analytes were hydrolyzed and extracted from 25-µL ethylenediaminetetraacetic acid-anticoagulated whole-blood spiked with isotope labeled 6-TG-C2N and 6-MMP-d3 internal standards.