Publications by authors named "H Kakishima"
Background: The Oncomine™ Dx Target Test based on next-generation sequencing has been approved for the screening of oncogenic mutations in advanced non-small-cell lung cancer patients.
Methods: We assessed the tissue sample factors that affect the success rate of Oncomine™ Dx Target Test companion diagnostics and the feasibility of using biopsy specimens for Oncomine™ Dx Target Test companion diagnostics in advanced non-small-cell lung cancer patients.
Results: Ninety-nine biopsy samples were subjected to genetic testing using the Oncomine™ Dx Target Test companion diagnostics to detect v-raf murine sarcoma viral oncogene homologue B1 mutations (Cohort 1), and 136 biopsy samples were examined using Oncomine™ Dx Target Test companion diagnostics for the detection of multiple oncogenic mutations (Cohort 2) between July 2018 and April 2020.
Article Synopsis
- Two boys, aged 23 months and 6 years, were found to have pediatric lung cancer due to tumors transmitted from their mothers during pregnancy.
- The first child showed spontaneous regression of some tumors, while the second experienced slow tumor growth, indicating possible immune responses to the transmitted cancer.
- Treatment with nivolumab, an immune checkpoint inhibitor, resulted in significant tumor regression in the first child, highlighting a promising approach for this rare cancer transmission.
Next-generation sequencing (NGS) of tumor tissue (ie, clinical sequencing) can guide clinical management by providing information about actionable gene aberrations that have diagnostic and therapeutic significance. Here, we undertook a hospital-based prospective study (TOP-GEAR project, 2nd stage) to investigate the feasibility and utility of NGS-based analysis of 114 cancer-associated genes (the NCC Oncopanel test). We examined 230 cases (comprising more than 30 tumor types) of advanced solid tumors, all of which were matched with nontumor samples.
View Article and Find Full Text PDFBackground: In this era of precision medicine, monitoring patients requires not only real time but also longitudinal sequence of samples at various time points. Based on this background, we focused on conditioned circumstances on fixation and storage for re-utilization of CTCs.
Materials: Instead of actual CTCs, Cell line (H1975) derived from lung cancer was used because of their scarceness of CTCs.