Genetic characterisation of Act1, the activator of a non-autonomous transposable element from Petunia hybrida.

The line W138 of Petunia hybrida has variegated flowers because it is homozygous for the mutable an1-W138 allele. Excision of the element, causing instability, depends on the presence of the activatorAct1. The previously characterised non-autonomous element dTph1 excises from the dfrC gene in response to Act1.

Petunia peroxidase a: isolation, purification and characteristics.

The fast-moving anionic peroxidase isoenzyme variant PRXa was purified from leaves of petunia (Petunia hybrida). Over 1300-fold purification was achieved by subjecting extracellular extracts to two sequential acetone precipitations and resuspending the pellets at pH 5.0 and pH 8.

Antigenic relationships between petunia peroxidase a and specific peroxidase isoenzymes in other Solanaceae.

A highly specific rabbit antiserum raised against peroxidase (PRXa) from petunia (Petunia hybrida) was used to investigate the antigenic relatedness of peroxidases in the Solanaceae. After SDS-PAGE of crude leaf extracts from a large number of species of this family, immunoblotting revealed that cross-reacting protein bands were present in all species tested. In order to determine whether these protein bands represent peroxidases, the peroxidase isoenzymes in thorn apple (Datura stramonium L.

Evidence for transposition in Petunia.

In Petunia interaction is described between a trans-regulatory genetic element Bi and responsive anthocyanin alleles, resulting in a variegated flower colour pattern. Crosses segregated white-flowering plants that had lost the Bi element and could be shown to contain a responsive allele by the colour pattern resulting from interaction with the standard variegated parent. A weak mutant of the Bi element is described.

Genetics of the peroxidase isoenzymes in Petunia : 11. Several loci involved in peroxidase synthesis.

The structural gene prxE, coding for a slow cathodic peroxidase in Petunia, has been located to chromosome II, linked to F1. The presence of two mobility alleles in Petunia hybrida can be ascribed to its hybrid descent. Some properties of peroxidase e are mentioned.