Publications by authors named "H J Maree"
Article Synopsis
- A team of over 180 researchers from more than 40 countries is addressing the issues related to "phantom agents," which are proposed pathogenic agents that are listed without concrete evidence of their existence.
- These phantom agents, identified only through symptoms and lacking proper isolates or genetic data, create obstacles for trade and plant certification, making effective detection and risk assessment difficult.
- The researchers recommend removing these agents from regulatory lists and updating standards in line with modern diagnostic methods to facilitate germplasm exchange and support global agriculture.
Article Synopsis
- Citrus tristeza virus (CTV) leads to economically damaging stem pitting in certain citrus varieties, but the mechanisms behind this damage are still not entirely understood.
- Researchers used infectious clones of CTV to investigate how stem pitting occurs in 'Duncan' grapefruit and 'Mexican' lime by replacing specific open reading frames (ORFs) that are thought to influence this condition.
- The study found that certain ORFs, particularly p18 and p23, were associated with increased stem pitting, and the analysis of metabolites revealed significant differences in compounds related to stress response, offering potential insights for improving CTV management techniques.
Validation of High-Throughput Sequencing (HTS) for Routine Detection of Citrus Viruses and Viroids.
Methods Mol Biol
December 2023
The management of plant diseases relies on the accurate identification of pathogens that requires a robust and validated tool in terms of specificity, sensitivity, repeatability, and reproducibility. High-throughput sequencing (HTS) has become the method of choice for virus detection when either a complete viral status of a plant is required in a single assay or if an unknown viral agent is expected. To ensure that the most accurate diagnosis is made from an HTS data analysis, a standardized protocol per pathosystem is required.
View Article and Find Full Text PDFRecent developments in high-throughput sequencing (HTS) technologies and bioinformatics have drastically changed research in virology, especially for virus discovery. Indeed, proper monitoring of the viral population requires information on the different isolates circulating in the studied area. For this purpose, HTS has greatly facilitated the sequencing of new genomes of detected viruses and their comparison.
View Article and Find Full Text PDFGrapevine leafroll disease (GLD) is a globally important disease that affects the metabolic composition and biomass of grapes, leading to a reduction in grape yield and quality of wine produced. Grapevine leafroll-associated virus 3 (GLRaV-3) is the main causal agent for GLD. This study aimed to identify protein-protein interactions between GLRaV-3 and its host.
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