Publications by authors named "H Huijgen"
Background: Efforts to identify therapies to treat hospitalised patients with COVID-19 are being continued. Alkaline phosphatase (AP) dephosphorylates pro-inflammatory adenosine triphosphate (ATP) into anti-inflammatory adenosine.
Methods: In a randomised controlled trial, we investigated the safety and efficacy of AP in patients with SARS-CoV-2 infection admitted to the ICU.
Article Synopsis
- Automated immunoassays (IAs) for measuring hormones like testosterone, cortisol, and free thyroxine (FT4) can be inaccurate in patients undergoing hemodialysis due to changes in their serum composition compared to healthy individuals.* -
- A study compared hormone levels in serum samples from both hemodialysis patients and healthy controls using a more reliable method (LC-MS/MS) alongside five different IAs, showing significant inaccuracies in the IAs, particularly for FT4 and testosterone in female patients.* -
- The findings highlight that hormone measurements using IAs may not be dependable in hemodialysis patients, indicating a need for healthcare professionals to take these discrepancies into account when interpreting results.*
Objective: Thyroid hormone measurements are often performed in pregnant women, as hypo- and hyperthyroidism during pregnancy can severely affect the fetus. Serum free thyroxine (fT4) measurements are well known for their analytical challenges, due to low serum concentrations and the subtle equilibrium between free and bound T4 (to thyroid-binding globulin (TBG), transthyretin and albumin). Pregnant women have high TBG concentrations due to an increase in human chorionic gonadotropin (hCG) and estrogen and lower albumin concentrations which change the equilibrium and may affect the validity of fT4 measurements in their samples.
View Article and Find Full Text PDFObjectives: Testosterone testing is relevant for evaluating castration adequacy and diagnosis of castration-resistant prostate cancer (PCa). However, the recommended testosterone cut-off of 1.7 nmol/L (50 ng/dL) to define adequate castration is based on consensus and not validated for the automated immunoassays (AIA) used in today's medical laboratories.
View Article and Find Full Text PDFObjectives: Multiple 25(OH)D assays have recently been aligned to improve comparibility. In this study we investigated the performance of these assays using both native single-donor sera with target values certified by a reference method as well as single donor sera from a heterogeneous patient population.
Design & Methods: 25(OH)D levels were measured in twenty reference samples (Ref!25OHD; Labquality, Finland) using five automated methods (Lumipulse, Liaison, Cobas, iSYS and Access) and one aligned ID-XLC-MS/MS method (slope: 1,00; intercept: 0,00; R=0,996).