[Variant creatine kinase isoenzymes: frequency and clinical significance (author's transl)].

To estimate the incidence of variant creatine kinase (CK)isoenzymes, 1,191 unselected patients were screened by means of CK:CK - MB ratio. When CK - MB was higher than 20% of the total CK in the immunoinhibition test, and a myocardial infarction could be excluded, a CK-isoenzyme electrophoresis was performed. We found macro-CK-BB-emia in 13 patients, but only three of them had elevated total CK activity.

Immunoinhibition and automated column chromatography compared for assay of creatine kinase isoenzyme MB in serum.

We examined sera from six different groups of patients for CK-MB activity by means of two commercially available tests, an immunoinhibition method (E. Merck) and the CK-MB test as used with the aca (Du Pont). In the first group of patients (suspicion of myocardial infarction) the correlation between the two methods was good: r = 0.

Evaluation of a monitor guided nephelometric system.

The most common parameters in the specific protein field, namely the immunoglobulins G, A and M were investigated on the recently developed Immuno Video Nephelometer System (IVNS). This system consists of the nephelometer, a microprocessor controlled program and a monitor screen, where instructions, standard curve and results are displayed. Scattered light of immuno-complexes is measured at equilibrium after incubation of prediluted antigen-antibody mixtures.