Publications by authors named "H Hisano"
Subsequent bacteremia developed in 14 % of patients with positive catheter tip cultures but concurrent negative blood cultures. The occurrence of subsequent bacteremia did not differ significantly by pathogens (Staphylococcus aureus, Gram-negative rods [GNR], and Candida spp.).
View Article and Find Full Text PDFThe hvbe2a mutations restore the starch-deficient phenotype caused by the hvisa1 and hvflo6 mutations in barley endosperm. The genetic interactions among starch biosynthesis genes can be exploited to alter starch properties, but they remain poorly understood due to the various combinations of mutations to be tested. Here, we isolated two novel barley mutants defective in starch BRANCHING ENZYME 2a (hvbe2a-1 and hvbe2a-2) based on the starch granule (SG) morphology.
View Article and Find Full Text PDFTransgenesis technologies, such as overexpression or RNA interference-mediated suppression, have often been used to alter the activity of target genes. More recently developed targeted genome modification methods using customizable endonucleases allow for the regulation or knockout mutation of target genes without the necessity of integrating recombinant DNA. Such approaches make it possible to create novel alleles of target genes, thereby significantly contributing to crop improvement.
View Article and Find Full Text PDFKnockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes many years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat simultaneously.
View Article and Find Full Text PDFArticle Synopsis
- - Genome editing in wheat shows potential for affecting genes across its three subgenomes, but current mutation rates need to be improved for effective research and trait modification.
- - By using a specific promoter from rice for sgRNA expression and applying a heat treatment during transformation, researchers found significant increases in Cas9-induced mutation rates.
- - The heat treatment at 30°C not only boosted the variety of mutations but also resulted in more integration sites without impacting the plant regeneration rates, which could enhance future studies on gene function and crop improvement.